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Molecular study of sortase enzyme and characterization of some virulence factors in streptococcus pyogenes
Other Title(s)
دراسة جزيئية لانزيم (sortase) وخصائص بعض عوامل الفوعة في المقيحة العقدية
Joint Authors
Razzaq, Muhammad Sabri A.
al-Jabburi, Rafah Fikri
Source
Issue
Vol. 9, Issue 1 (31 Mar. 2012), pp.74-83, 10 p.
Publisher
University of Babylon College of Medicine
Publication Date
2012-03-31
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Abstract AR
من مجموع 90 مسحة بلعومية قد تم الحصول عليها من مرضى تتراوح أعمارهم (3-63) سنة يعانون من إلتهاب البلعوم من المراجعين إلى مستشفى الحلة الجراحي (وحدة ENT) للفترة من أكتوبر 2010 إلى آذار 2011، تم عزل ستة عزلات فقط من بكتريا المسبحيات القيحية (Streptococcus pyogenes).
ظهر أن أربعة عزلات من بكتريا المسبحيات القيحية لها القابلية على تحليل الفايبرين من خلال أنتاجها لانزيم Streptokinase.
كما أظهرت بعض العزلات أن فعالية أنزيم Streptokinase تظهر واضحة بعد ثلاثة ساعات من الحضن و أن الخثرة (clot) تبدأ بالتقلص بعد 10-16 ساعة و تختفي تماما بعد 24 ساعة.
تم التحري عن فعالية أنزيم Hyaluronidase و قد وجد أن 50 % من العزلات أعطت نتائج موجبة من خلال تكوين منطقة شفافة حول المستعمرة و هذا يعتبر مؤشر على وجود فعالية لهذا الأنزيم.
تم استخدام بادئات نوعية (primers) في تقنية تفاعل البلمرة المتسلسل (PCR) للتحري عن الجينات الخاصة بأنزيمات Sortase (C A, B) و قد أظهرت النتائج بامتلاك كافة العزلات لجين Sortase A في حين عزلتين فقط أظهرتا احتواءها لجين Sortase B و Sortase C.
على أية حال فأن نتائج PCR أظهرت بأن عزلتين فقط تمتلك الجينات للأنواع الثلاثة من أنزيم Sortase.
Abstract EN
This study included 90 throat swabs collected from patients suffering from acute pharyngitis who attending to AL-Hilla General Teaching Hospital (ENT unit) during the period from October 2010 to January 2011.
The age of the patients ranged from (3 to 63) years.
Only six isolates of Streptococcus pyogenic were obtained.
It was observed that only four isolates had the fibrinolysis activity through the production of streptokinase enzyme, while two isolates failed to lyse fibrin, also the present study showed that streptokinase activity became apparent after 3 hours of incubation and clot began to decrease after 10-16 hours and disappeared completely after 24 hours of incubation.
Hyaluronidase activity was investigated, it was found that 50 % (3) of GAS isolates give a positive result via the formation of a hallo around the colony and this is an indicator of Hyaluronidase activity.
Specific primers for PCR techniques were used for the detection of sorties enzymes (A, B and C).
A SORTIE A was found existed in all GAS strains and this enzyme was designated as housekeeping enzyme.
Sorties B and C were also detected, it was found that only two isolates were positive for both sorties B and C genes.
However, only two isolates were found to have all these three enzymes whereas the others contained only the housekeeping one.
American Psychological Association (APA)
Razzaq, Muhammad Sabri A.& al-Jabburi, Rafah Fikri. 2012. Molecular study of sortase enzyme and characterization of some virulence factors in streptococcus pyogenes. Medical Journal of Babylon،Vol. 9, no. 1, pp.74-83.
https://search.emarefa.net/detail/BIM-304503
Modern Language Association (MLA)
Razzaq, Muhammad Sabri A.& al-Jabburi, Rafah Fikri. Molecular study of sortase enzyme and characterization of some virulence factors in streptococcus pyogenes. Medical Journal of Babylon Vol. 9, no. 1 (2012), pp.74-83.
https://search.emarefa.net/detail/BIM-304503
American Medical Association (AMA)
Razzaq, Muhammad Sabri A.& al-Jabburi, Rafah Fikri. Molecular study of sortase enzyme and characterization of some virulence factors in streptococcus pyogenes. Medical Journal of Babylon. 2012. Vol. 9, no. 1, pp.74-83.
https://search.emarefa.net/detail/BIM-304503
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 82-83
Record ID
BIM-304503