Characterization of vero toxigenic escherichia coli isolated from slaughtered small ruminants and slaughterhouses in southern Jordan
Other Title(s)
تمييز الاشريشيا القولونية منتجه سموم الفيرو المعزولة من ذبحيات المجترات الصغيرة و المسالخ في جنوب الأردن
Dissertant
Thesis advisor
al-Tarawinah, Khalid Ahmad Jafar
Comitee Members
Abd al-Ghani, Adil Hasan Mahmud
Kanan, Ghassan Jaddu
al-Majali, Ahmad
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2007
English Abstract
The emergence of Verotoxin-producing Escherichia coli (VTEC) as zoonotic food-borne pathogens has become a public health concern because of its life threatening human diseases.
Rapid identification is important for meat hygiene management and prompts epidemiological investigations.
This study was conducted to determine the prevalence rate of VTEC in slaughtered sheep and goats and to evaluate the contamination rate of VTEC in slaughterhouses and butcher shops in southern Jordan.
Among collected animals' fecal samples (n =402) and slaughterhouses/butcher shops samples (n = 57) about 201 and 33, respectively, E.
coli isolates were characterized by multiplex PCR reaction for detection of stx1, stx2, eaeA and E-hly A virulent genes.
Throughout the study, twenty six virulent E.
coli isolates had been characterized by mPCR to 7 different virulent patterns, stx1, stx1+stx2, stx1+eaeA, stx1+E-hlyA, stx1+eaeA+E-hlyA, eaeA and E-hlyA.
It was found that VTEC comprised 6.4% and 21% of the total E.
coli isolates from slaughtered small ruminants and slaughterhouses/butcher shops, respectively.
In a total of 20 characterized VTEC, the proportion of stx1 and stx2 was 19 : 1.
Through out the study, it was founded that characterized complex VTEC (contain eaeA and/or E-hlyA) possessed 3 virulence patterns including, (VTEC) stx1+eaeA, (VTEC/EHEC) stx1 + E-hlyA and (VTEC/EHEC) stx1+eaeA+E-hly A in a percentage of 30%, 25% and 5 %, respectively, to the total VTEC isolates.
Only one VTEC isolates characterized as E.
coli O157, highly pathogenic strain.
It was isolated in a percentage of 0.4% to the total E.
coli isolates with virulent pattern, stx1, eaeA and E-hlyA.
Isolated VTEC showed highly sensitivity to gentamicin and co-trimoxazole and highly resistance ability for tetracycline and ampicillin.
Main Subjects
Topics
No. of Pages
82
Table of Contents
Table of contents.
Abstract.
Chapter one : theoretical background.
Chapter two : literature review.
Chapter three : design and methodology.
Chapter four : findings and discussions.
Chapter five : summary and recommendations.
References.
American Psychological Association (APA)
al-Turah, Nafi M.. (2007). Characterization of vero toxigenic escherichia coli isolated from slaughtered small ruminants and slaughterhouses in southern Jordan. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-304607
Modern Language Association (MLA)
al-Turah, Nafi M.. Characterization of vero toxigenic escherichia coli isolated from slaughtered small ruminants and slaughterhouses in southern Jordan. (Master's theses Theses and Dissertations Master). Mutah University. (2007).
https://search.emarefa.net/detail/BIM-304607
American Medical Association (AMA)
al-Turah, Nafi M.. (2007). Characterization of vero toxigenic escherichia coli isolated from slaughtered small ruminants and slaughterhouses in southern Jordan. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-304607
Language
English
Data Type
Arab Theses
Record ID
BIM-304607
