TP53 over expression in colorectal carcinoma by immunohistochemistry

Abstract EN

Fifty paraffin blocks of colorectal carcinomas cases included in this study were randomly selected from January 2007 to Juan 2007 from the histopathologically department in Al-Sadr Teaching Hospital.

Of the fifty paraffin blocks of the studied colorectal carcinomas' cases ; 29 cases (58 %) were males and 21 cases (42 %) were females.

Their ages range was (16-80) years with an overall mean age (56.44 ± 13.558) years, (58 %) cases in distal colon (26 %) cases were rectal, well differentiated carcinoma in (40 %) cases, their stages by Dukes they were (36 %) Dukes C followed by (30%) Dukes B then (18 %) Dukes D and (16 %) Duke's A.

Fifty cases were submitted for immunohistochemical stain for P53 antibody, 39 cases (78 %) were positive, and positive cases classified as weak positive in 18 cases (36 %) and strong positive in 21cases (42 %), also as faint staining pattern in 15 cases (38.4 %) and dense staining pattern in 24 cases (61.6 %).TP53 expression was more in distal colon cases (82.7 %), higher stages Dukes C (83.3) and D ( 88.8%) and poorly differentiated cancer (94.7%). With no significant correlation of TP53 expression and site and stage of tumor but with significant relationship with grade of tumor