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Expression of bax mrna in patients with colorectal carcinomas using insitu hybridization
Other Title(s)
التعبير عن الحامض النووي الرايبوزي الناقل ل Bax في النسيج المطمور بالبرافين المأخوذ من مرضى سرطان القولون باستخدام تقنية التهجين الموضعي
Joint Authors
Abd al-Muhaymin, Nidal
Afnan, Faizah
Lelo, Abd al-Amir
Source
Iraqi Journal of Biotechnology
Issue
Vol. 10, Issue 1 (30 Jun. 2011)9 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2011-06-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Topics
Abstract AR
هدفت الدراسة إلى التحري عن التعبیر عن Bax باستعمال تقنیة التهجین الموضعي للتعبیر عن الحامض النووي الرایبوزي الناقل في مقاطع نسیجیه مطمورة بالبارافین لمرضى مصابین بسرطان القولون و كان عددهم 43 مريض, إضافة إلى 26 مقطعا نسیجیي لمرضى مصابین بفرط التنسج أو ورم غدي كما شملت الدراسة 35 مقطع نسیجي طبیعي للقولون.
بینت النتائج أن التعبیر عن هذا المعلم كان موجبا في 28 عینة من مرضى السرطان و أنه لا توجد هنالك علاقة بین موقع الورم و التعبیر عن المعلم.
أما نتائج المرضى المصابین بفرط التنسج أو ورم غدي فقد بینت بأن المعلم موجود في 19 حالة، في حین كانت النتائج سالبة للـ 35 حالة التي أخذت من أنسجة سلیمة.
كما بینت النتائج بأن التعبیر عن هذا المعلم فى المرضى المصابین بفرط التنسج أو ورم غدي كان أعلى منه مما هو علیه فى مرضى سرطان القولون و بمستوى معنوي (p≤0.001).
Abstract EN
The study investigated the expression of Bax mRNA using in situ hybridization technique in the paraffin sections from 43 biopsies of patients with cancers of colorectal (CRC), 26biopsies from patients with hyperplastic polyps and adenomas (CRHPA) and 35-colon normal tissue (CRN).
This investigation correlated the expression patterns with tumor histopathologically type, site of the tumor in colorectal cancer patients.
Results indicated that in colorectal cancer (CRC) patients’ Bax mRNA expression was detected in 28 of 43.
Patients with colorectal cancer were grouped according to the site of the tumor into proximal, distal and rectal site.
There was no association between site of tumor and marker detection and no association with the histopathologically type.
In colorectal hyperplastic polyp and adenoma specimens, Bax mRNA expression was detected in 19 of 26.
For the determination of the differences in marker expressions in relation to the histopathologically type, results showed highly significant in the tubular and villous adenoma expression with P value < 0.01.
The expression of this marker in relation to the site of the tumor had been studied and showed no significant difference in the expression.
In colorectal normal specimens Bax mRNA expression showed significant high expression.
While in the (CRN) results were below the used cutoff value for each one of them and regarded as negative.
In conclusions, Bax, mRNA expression has high level in CRHPA than in CRN tissue with P value <0.001.
Also, its expression in CRC is more than in CRN tissue with a P value < 0.001.
American Psychological Association (APA)
Lelo, Abd al-Amir& Abd al-Muhaymin, Nidal& Afnan, Faizah. 2011. Expression of bax mrna in patients with colorectal carcinomas using insitu hybridization. Iraqi Journal of Biotechnology،Vol. 10, no. 1.
https://search.emarefa.net/detail/BIM-307994
Modern Language Association (MLA)
Abd al-Muhaymin, Nidal…[et al.]. Expression of bax mrna in patients with colorectal carcinomas using insitu hybridization. Iraqi Journal of Biotechnology Vol. 10, no. 1 (2011).
https://search.emarefa.net/detail/BIM-307994
American Medical Association (AMA)
Lelo, Abd al-Amir& Abd al-Muhaymin, Nidal& Afnan, Faizah. Expression of bax mrna in patients with colorectal carcinomas using insitu hybridization. Iraqi Journal of Biotechnology. 2011. Vol. 10, no. 1.
https://search.emarefa.net/detail/BIM-307994
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references.
Record ID
BIM-307994