Uptake of chromium by bacteria isolated from As-Samra wastewater treatment plant

Other Title(s)

امتصاص الكروم بواسطة بكتيريا معزولة من محطة تنقية الخربة السمراء

Dissertant

Qokazeh, Duha Ali

Thesis advisor

Irshaid, Fawzi
al-Tarawinah, Khalid Ahmad

Comitee Members

Jaran, Adnan S.
Khulayfat, Khalid Muhammad
Qar, Janti S.

University

Al albayt University

Faculty

Faculty of Sciences

Department

Department of Biological Sciences

University Country

Jordan

Degree

Master

Degree Date

2010

English Abstract

Chromium (Cr), one of heavy metals, Cr is widely used in chrome-plating, leather tanning, paint pigments, wood treatment.

Even at low concentration, accumulation of Cr in the environment is a serious concern for humans, animal and plant development, growth and health.

Therefore, Cr level must be monitored and removed from water resources.

Thus, the presence of high concentration of Cr in wastewater in Jordan is threatening to water supplies, public health and agriculture.

Therefore, the main goal of our study was to isolate and identify bacterial strains from As-Samra Wastewater Treatment Plant (SWTP) that can deal with uptake Cr at highly concentration. Samples were collected from SWTP.

Morphological and biochemical characterization have been employed to identify the isolated bacterial strains.

Ability of these isolates to uptake Cr ions was also measured by flame atomic absorption spectrometry technique.

The ability of the selected species to uptake, adsorption, and bio sorption of Cr were tested in under various conditions, such as different nitrogen and carbon sources and various types of media.

Plasmid was also isolated from this species.

The possibility that this plasmid might responsible for this phenotype was also examined. Both biochemical and morphological analysis revealed that these ten bacterial isolates identified as Acinetobater calcoaceticus, Klebsiella oxytoca, Pasteur Ella multocida, Proteus vulgaris, E.

cloacae, Escherichia coli, Shigella sp.

Vibrio fluvialis, Burkholderia cepacia, Citrobacter freundii.

Ability of these isolates to uptake Cr ions was also tested.

E.

cloacae isolate had the highest Cr+ uptake (40.68 ppm/g wet weight) when compared to other isolates.

Therefore, E.

cloacae species were selected for further analysis.

The Cr concentrations of about 90 ppm and above markedly inhibited the growth in Cr concentration-dependent manners.

E.

cloacae exhibit different responses to the different carbon and nitrogen sources.

Sucrose, disaccharide, showed the highest uptake of Cr by E.

cloacae, when compared other carbon sources, such maltose and glucose.

In addition, the ammonium nitrate had the highest uptake of Cr by E.

cloacae, this was followed by tryptone, and the least uptake was observed in ammonium chloride.

The removal efficiency of E.

cloacae was 67.91 % when it cultured in 40 mg Cr / L- Nutrient broth.

Plasmid curing of E.

Cloacae with ethidium bromide was enhance the uptake and increased it ^4 fold when it compared with parental E.

cloacae cells; so the possibility that this plasmid might responsible for the Cr resistance while may be the chromosome responsible for the Cr uptake. In conclusion, our results indicated that E.

cloacae species was efficient in removal of Cr from nutrient broth compared to other tested microorganisms.

This finding suggests the possible use of this species in removal of heavy metals such as Cr from water resources.

Main Subjects

Biology

Topics

• Jordan
• Sewage
• Water
• Sewage.

No. of Pages

73

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-310862