The protective role of vitamin C against lead acetate-induced hepatotoxicity and ephrotoxicity in female rats and their pups
Other Title(s)
الدور الوقائي لفيتامين (ج) ضد التسمم الكبدي و الكلوي بسبب خلات الرصاص في إناث الجرذان البيض و مواليدها
Dissertant
Thesis advisor
Comitee Members
Ismail, Nazim J.
Jumah, Hamad Jandari
Wahid, Intisar Numman
Mahud, Abd al-Karim Salim
University
Salahaddin University-Hawler
Faculty
College of Science
Department
Biology Department
University Country
Iraq
Degree
Master
Degree Date
2010
English Abstract
The purpose of the present investigation is to study the effect of lead (as lead acetate) on the liver and kidney of female rats and the embryonic development of these two organs in their one day old pups and the protective role of Vitamin C against these effects.
On the way to achieve this purpose and to explain the histological and teratological alterations, several other hematological, biochemical and morphological parameters were included.
Sixty female albino rats were used and randomly divided into six groups, ten rats in each group.
The first group has considered as control group and has given only tap water.
The second group has given gm Vitamin C / Kg b.w.
in the drinking water.
The third and fourth groups were given and % lead acetate (PbAc) respectively in the drinking water.
The fifth and sixth groups were given % lead acetate plus Vitamin C and % lead acetate plus Vitamin C respectively in the drinking water.
At the first day of birth, all the mothers and pups were dissected after weighing and the liver and kidney were removed after collecting the blood.
The sera were prepared for the biochemical analysis.
The hematological study has showen non significant decrease of WBC count in the lead acetate groups in comparison to the control group, while vitamin C when administrated the PbAc groups has shown non significant increase in WBC count.
Similar protective result was recorded with regards to the decreased RBC count especially the pb plus vitamin C in which non significant increase was detected.
Hemoglobin level and blood platelets count have shown variable results in the studied groups.
The biochemical study showed a non significant decrease in the GSH level in both doses of lead acetate with a significant and non significant increase in the and PbAc respectively after administration of Vitamin C to them.
Both doses of lead acetate have caused an increase in serum MDA level but Vitamin C have succeeded in decreasing this level after given it to the PbAc treated groups.
The quantitative estimation of lead in liver, kidney and placenta has shown a significant increase of lead in the higher dose of lead acetate treated group, while this quantity was lowered in the lead acetate vitamin C combination groups.
Both doses of lead acetate have caused significant increase in the number of dead hepatocytes in female (the mothers) liver.
The main mode of cell death as revealed by EM was the programmed cell death, apoptosis.
Vitamin C has shown a protective role by decreasing this number of dead cells in the combination groups.
In the lead acetate groups, several histological and ultrastructural alterations were detected but vitamin C when administrated in combination with the lead acetate doses has approximately protected the liver against this hepatotoxic effect with respect to both lead acetate doses.
On the other hand, the liver of pups has shown dose dependent effects and the main effect was the fatty changes.
Treating the mothers with vitamin C in addition to lead acetate has lowered these effects.
With respect to kidney, both doses of lead acetate have caused nephrotoxic effects in the kidney of the female rats (the mothers), but the higher dose has caused a significant increase of dead proximal convoluted tubules (PCT) cells in comparison to the all treatments, while vitamin C when administrated to this higher dose, caused non significant decrease of the number of these dead cells.
The histological alterations that have been caused by both doses of lead acetate have been approximately disappeared in the combination groups.
The most interesting feature of nephrotoxicity caused by lead acetate is the accumulation of this metal in the kidney tissue of the exposed rats as revealed by EM figures.
Treating female rats (the mothers) with lead acetate at date of gestation has caused disturbance in glomerulugenesis and tubulogenesis of pup kidney.
Crossing of metal molecules to kidney tubules has been suggested and this has been concluded depending on EM figures.
Vitamin C has shown a protective role with respect to the previous alterations and has approximately showed a normal developmental stages of glumerulugenesis and tubulogenesis in pup kidney.
Main Subjects
Topics
No. of Pages
122
Table of Contents
Table of contents.
Abstract.
Chapter One : introduction.
Chapter Two : literature review.
Chapter Three : materials and methods.
Chapter Four : results and discussion.
Conclusions and recommendations.
References.
American Psychological Association (APA)
Abd al-Qadir, Shang Ziyad. (2010). The protective role of vitamin C against lead acetate-induced hepatotoxicity and ephrotoxicity in female rats and their pups. (Master's theses Theses and Dissertations Master). Salahaddin University-Hawler, Iraq
https://search.emarefa.net/detail/BIM-310911
Modern Language Association (MLA)
Abd al-Qadir, Shang Ziyad. The protective role of vitamin C against lead acetate-induced hepatotoxicity and ephrotoxicity in female rats and their pups. (Master's theses Theses and Dissertations Master). Salahaddin University-Hawler. (2010).
https://search.emarefa.net/detail/BIM-310911
American Medical Association (AMA)
Abd al-Qadir, Shang Ziyad. (2010). The protective role of vitamin C against lead acetate-induced hepatotoxicity and ephrotoxicity in female rats and their pups. (Master's theses Theses and Dissertations Master). Salahaddin University-Hawler, Iraq
https://search.emarefa.net/detail/BIM-310911
Language
English
Data Type
Arab Theses
Record ID
BIM-310911
