A simple co-culture system for generation of embryonic stem like cells from testis
Joint Authors
Bojnordi, Maryam Nazm
Movahedin, Mansoureh
Tiraihi, Taki
Javan, Muhammad
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 14, Issue 12 (31 Dec. 2012)14 p.
Publisher
Publication Date
2012-12-31
Country of Publication
United Arab Emirates
No. of Pages
14
Main Subjects
Topics
Abstract EN
Background : New research proposes the pluripotency of spermatogonial cells obtained from testis.
These spermatogonia-derived stem cells are called embryonic stem like cell that express embryonic stem cell markers and differentiate to the three germ layers. Objectives : The aim of this study was the generation of embryonic stem like cells from neonatal mouse testis. Materials and Methods : Testis cells were collected from neonatal mouse.
After decapsulation, testis was mechanically dissected and dissociated via a two-step mechanical and enzymatic digestion.
The spermatogonia and sertoli cells were cultured together in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15 % FBS and LIF.
Before one week, several small spermatogonia colonies were observed on top of the monolayer of sertoli cells.
These colonies were passaged every four days.
ES Like cells colonies that resembled ES cell was appeared within 2-3 weeks (at passages 5).
Real time PCR was performed to analyze the expression of a subset of pluripotency markers, as well as germ cell-specific genes.
ES Like cells were confirmed with SSEA1, SOX2 and Oct4 immunofluorescence stainng as pluripotency markers. Results : Results showed that at fifth passages, the pluripotency genes; Nanog and c-myc has significant increase in ES Like cells in compare with spermatogonia cells, whereas the spermatogonial markers ; Stra8, mvh, and piwill2 became downregulated.
In addition to these pluripotency genes, the ES cell marker SSEA-1, SOX2 and Oct4 was expressed in the ES-like cells, similar to ES cells. Conclusions : These researh indicates pluripotency evidence of ES-like cells derived from testis.
ES-like cells shows some molecular characteristics with embryonic stem cells.
American Psychological Association (APA)
Bojnordi, Maryam Nazm& Movahedin, Mansoureh& Tiraihi, Taki& Javan, Muhammad. 2012. A simple co-culture system for generation of embryonic stem like cells from testis. Iranian Red Crescent Medical Journal،Vol. 14, no. 12.
https://search.emarefa.net/detail/BIM-310944
Modern Language Association (MLA)
Bojnordi, Maryam Nazm…[et al.]. A simple co-culture system for generation of embryonic stem like cells from testis. Iranian Red Crescent Medical Journal Vol. 14, no. 12 (Dec. 2012).
https://search.emarefa.net/detail/BIM-310944
American Medical Association (AMA)
Bojnordi, Maryam Nazm& Movahedin, Mansoureh& Tiraihi, Taki& Javan, Muhammad. A simple co-culture system for generation of embryonic stem like cells from testis. Iranian Red Crescent Medical Journal. 2012. Vol. 14, no. 12.
https://search.emarefa.net/detail/BIM-310944
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references.
Record ID
BIM-310944