Biochemical characterizations and genotyping by RAPD-PCR analyses of malassezia spp. from pityriasis versicolor and seborrhoeic dermatitis patients
Author
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 14, Issue 11 (30 Nov. 2012)6 p.
Publisher
Publication Date
2012-11-30
Country of Publication
United Arab Emirates
No. of Pages
6
Main Subjects
Topics
Abstract EN
Background : To investigate the biochemical-physiological characteristics and genetic variations of Malassezia species isolated from pityriasis versicolor and seborrhoeic dermatitis patients.
Objectives : The aims of the present study were : firstly, to determine the distribution of Malassezia species in PV and SD in South-west Asia (Iran) and secondly, to analyze the DNA profile by RAPD-PCR of Malassezia species isolated from patients studied.
Materials and Methods : Twenty two pityriasis versicolor and seborrhoeic dermatitis outpatients at Razi Hospital of Tehran University were included in this study.
The samples were cultured and identified for Malassezia spp.
by the conventional techniques based on the morphological, biochemical and physiological characteristics.
Random amplification of polymorphic DNA polymerase chain reaction (RAPDPCR( was applied for genetic variations of the isolates.
Results : M.
furfur was the prevalent species isolated from pityriasis versicolor either alone (44 / 4 %) or in combination with M.
globosa or M.
sympodialis (44 / 4 %) ; while M.
globosa was most frequently isolated from seborrhoeic dermatitis either alone (38/5 %) or combination with M.
furfur (7 / 7 %).
The RAPD analysis among Malassezia isolates was counteracted from 238 amplified products in 81 separable positions and indicated that all Malassezia isolates were composed of nine genetically distant groups.
Conclusions : We found different physiological characteristics and genetic variations of Malassezia isolates from all patients.
Moreover, RAPDPCR can be used as a powerful tool in epidemiological investigation of dermatological disorders associated with Malassezia species such as pityriasis versicolor and seborrhoeic dermatitis.
American Psychological Association (APA)
Eidi, Samaneh. 2012. Biochemical characterizations and genotyping by RAPD-PCR analyses of malassezia spp. from pityriasis versicolor and seborrhoeic dermatitis patients. Iranian Red Crescent Medical Journal،Vol. 14, no. 11.
https://search.emarefa.net/detail/BIM-310957
Modern Language Association (MLA)
Eidi, Samaneh. Biochemical characterizations and genotyping by RAPD-PCR analyses of malassezia spp. from pityriasis versicolor and seborrhoeic dermatitis patients. Iranian Red Crescent Medical Journal Vol. 14, no. 11 (Nov. 2012).
https://search.emarefa.net/detail/BIM-310957
American Medical Association (AMA)
Eidi, Samaneh. Biochemical characterizations and genotyping by RAPD-PCR analyses of malassezia spp. from pityriasis versicolor and seborrhoeic dermatitis patients. Iranian Red Crescent Medical Journal. 2012. Vol. 14, no. 11.
https://search.emarefa.net/detail/BIM-310957
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references.
Record ID
BIM-310957