Burden and typing of rotavirus group A in children with acute gastroenteritis in Shiraz, Southern Iran
Joint Authors
Kargar, M.
Jafarpour, T.
Najafi, A.
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 14, Issue 9 (30 Sep. 2012)9 p.
Publisher
Publication Date
2012-09-30
Country of Publication
United Arab Emirates
No. of Pages
9
Main Subjects
Topics
Abstract EN
Background : Human Rotavirus is a significant cause of severe gastroenteritis in infants and young children worldwide.
In recent years, Rotavirus genotyping by RT-PCR has provided valuable information about the diversity of Rotaviruses circulating worldwide.
The purpose of the present study is to monitor the prevalence of the different G types of Rotaviruses circulating in Shiraz, Southern Iran and detect any uncommon or novel types. Methods : Methods : During the period from December 2007 to November 2008, a total of 138 stool samples were collected from children less than 5 years old who were hospitalized for acute gastroenteritis.
Rotavirus-associated diarrhea was investigated in fecal specimens with enzyme immunoassays (EIA).
Rotavirus-positive specimens were typed by the Nested RT-PCR and by using different types of specific primers. Results : Out of the 138 collected samples, 34.78 % (48 cases) tested positive for Rotavirus.
The frequency of G1, G2 and G4 types was 6.25 %, 2.08 % and 27.08 %, respectively.
Mixed and non-typeable infections were detected in 33.34 % and 31.25 % of hospitalized children with acute diarrhea, respectively.
This is the first time mixed Rotavirus infections with G1 / G3 have been reported in Iran. Conclusion : The high frequency of Rotavirus detection indicates the severity and the burden of Rotavirus disease may be able to reduce through the implementation of an effective vaccine and continual surveillance for the detection of Rotavirus genotypes circulating in other regions of Iran. Regarding to the noticeable frequency of non-typeable and mixed infections, it is suggested to use the other specific primers and further studies to detection of other novel and unusual types.
American Psychological Association (APA)
Kargar, M.& Jafarpour, T.& Najafi, A.. 2012. Burden and typing of rotavirus group A in children with acute gastroenteritis in Shiraz, Southern Iran. Iranian Red Crescent Medical Journal،Vol. 14, no. 9.
https://search.emarefa.net/detail/BIM-311010
Modern Language Association (MLA)
Kargar, M.…[et al.]. Burden and typing of rotavirus group A in children with acute gastroenteritis in Shiraz, Southern Iran. Iranian Red Crescent Medical Journal Vol. 14, no. 9 (Sep. 2012).
https://search.emarefa.net/detail/BIM-311010
American Medical Association (AMA)
Kargar, M.& Jafarpour, T.& Najafi, A.. Burden and typing of rotavirus group A in children with acute gastroenteritis in Shiraz, Southern Iran. Iranian Red Crescent Medical Journal. 2012. Vol. 14, no. 9.
https://search.emarefa.net/detail/BIM-311010
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references.
Record ID
BIM-311010