Rapid detection of escherichia coli O157 : H7 by fluorescent amplification-based specific hybridization (FLASH) PCR
Joint Authors
Khatami, F.
Haydari, M.
Khatami, M.
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 14, Issue 9 (30 Sep. 2012)5 p.
Publisher
Publication Date
2012-09-30
Country of Publication
United Arab Emirates
No. of Pages
5
Main Subjects
Abstract EN
Background : Escherichia coli O157 : H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome in humans. Methods : We developed fluorescent amplification-based specific hybridization (FLASH–PCR) assay to detect the Stx-encoding gene Stx-1 of E.
coli O157 : H7. Result : PCR product of 336 bp was successfully amplified in a FLASH–PCR. Conclusion : As rapid detection and confirmation of the presence of E.
coli O157 : H7 are of importance for the medical, food, and water industries, FLASH-PCR is one of selective methods for detection of E.
coli O157 : H7.
American Psychological Association (APA)
Khatami, F.& Haydari, M.& Khatami, M.. 2012. Rapid detection of escherichia coli O157 : H7 by fluorescent amplification-based specific hybridization (FLASH) PCR. Iranian Red Crescent Medical Journal،Vol. 14, no. 9.
https://search.emarefa.net/detail/BIM-311017
Modern Language Association (MLA)
Khatami, F.…[et al.]. Rapid detection of escherichia coli O157 : H7 by fluorescent amplification-based specific hybridization (FLASH) PCR. Iranian Red Crescent Medical Journal Vol. 14, no. 9 (Sep. 2012).
https://search.emarefa.net/detail/BIM-311017
American Medical Association (AMA)
Khatami, F.& Haydari, M.& Khatami, M.. Rapid detection of escherichia coli O157 : H7 by fluorescent amplification-based specific hybridization (FLASH) PCR. Iranian Red Crescent Medical Journal. 2012. Vol. 14, no. 9.
https://search.emarefa.net/detail/BIM-311017
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references.
Record ID
BIM-311017
