Antibacterial effect of plant oil extracts and simvastatin treatment of burn wounds infected with pseudomonas aeruginosa and staphylococcus aureus in albino rats

Other Title(s)

التأثير المضاد البكتيري لمستخلصات الزيوت النباتية و معالجة الحروق الملوثة بـ aeruginosa Pseudomonas و Staphylococcus aureus بواسطة السمفاستاتين في الجرذان البيض

Dissertant

Ali, Shahnaz Burhan

Thesis advisor

Salih, Faridun Abd al-Qadir

Comitee Members

Mustafa, Inam Ahmad
Mahmoud, Bakhtiar M.
Mansur, Isam Y.

University

Salahaddin University-Hawler

Faculty

College of Science

Department

Biology Department

University Country

Iraq

Degree

Master

Degree Date

2011

English Abstract

-One hundred swabs were collected from patients suffering from burn wound infections admitted to Emergency Management Centre in Erbil city in Kurdistan-Iraq.

In this study, 60 isolates were identified as Pseudomonas aeruginosa, representing 39.21%, while Staphylococcus aureus were 47 isolates, represents 30.71%.

On other hand Klebsiella pneumonia, representing 16.99%, Proteus mirabilis 3.26%, Serratia sp.

and Streptococcus fecalis 2.61%, Escherichia coli, Enterobacter sp.

and Staphylococcus epidermities represent 1.30%, and Acintobacter sp.

represents 0.65% out of 100 samples. The more resistant strain of Pseudomonas aeruginosa and Staphylococcus aureus were screened for in vitro antimicrobial effect of virgin olive, cocconut and bitter almond oil and Simvastatin (SIM) which is a lipid lowering drug.

The antibacterial and anti-inflammatory effects of Simvastatin on burn wound infection with multiresistant Pseudomonas aeruginosa and methicillin resistant Staphylococcus aureus, and wound healing progress in rat model through bacteriological examination, nacked eye observation, and histological sections of skin and hematological parameters were studied in vivo. The age of patients ranged from (10- 47), the majority of burn patients aged between 17-30 years, representing 54% and most of them were females 67%, while males represented 33%. The studied bacterial isolates were screened for their susceptibility to different antibiotics.

P.

aeruginosa isolates were moderately resistant to Gentamicin 55% whereas they showed high resistant to Ciprofloxacin 70%, Cefotaxime 80% and 85% for Carbenicillin, Chloramphenicol, and Lincomycin.

Amoxicillin/Clavulanic acid, Cephalothin, Kanamycin and Rifampin resistance rates were 90%.

The highest resistant rate was for both Ofloxacin and Doxycycline (95.5%) and no isolate has been found to be sensitive to all antibiotics.

The sensitivity Pseudomonas aeruginosa was higher to newer generation of antibiotics as is evident by only 5% resistance to Imipenem and 30% resistance to Amikacin and Azteronam. Staphylococcus aureus showed higher resistant rate of about 83.3% to Cefotaxime and Penicillin G.

The most effective antibiotic was found to be Cefoxitin since the resistance rate was 30%.

Oxacillin is used instead of methicillin to determine methicillin resistant Staphylococcus aureus (MRSA), which was found to be 90%. In vitro determination of antimicrobial activity of extracted plant oils showed that there was inhibitory effect on the growth of P.

aeruginosa and S.

aureus by the different volumes of oils.

The virgin olive oil showed higher inhibition of bacterial growth of about 23-25mm against P.

aeruginosa and 26-29mm against S.

aureus when compared to coconut and bitter almond oils. On the other hand, in vitro determination of antimicrobial activity of SIM showed that SIM had higher inhibitory effect in using both agar and disc diffusion methods against multidrugs resistant P.

aeruginosa and MRSA than extracted plant oils.

SIM at 500μl/ml in agar diffusion method showed a significant antimicrobial effect against P.

aeruginosa (mean 28.6 ± 0.7mm) and (mean 31.6 ± 0.6mm) against MRSA.

While the disc diffusion method showed (mean 24.2 ± 0.9mm) against P.

aeruginosa and (mean 25.6±0.7mm) against MRSA. In vivo determination of antibacterial and anti-inflammatory activity of SIM against more resistant isolate of each P.

aeruginosa and MRSA applied to burned dorsal skin area of female albino rats showed a reduction in these infectious agents among Simvastatin treated groups especially in locally treated group 8. Macroscopical examinations revealed redness and pus production in non treated group rats more than in treated groups and they were physically less active when compared with pretreatment activity.

The histological sections of skin of SIM groups showed a significant improvement in healing and skin layers differentiation when compared with no SIM treated infected skin wounds. The results of hematological parameters showed that the level of inflammatory cells was down regulated by administration or topical application of SIM.

Main Subjects

Biology

Topics

• Chemotherapy
• Antibacterial agents
• Bacterial diseases
• Burns

No. of Pages

140

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-311103