Attachment of embryonic stem cells-derived cardiomyocytes in cultispher-S microcarriers by using spinner flask
Joint Authors
Akasha, Abd al-Rahman A.
Elouz, Abd al-Rahim A.
Source
Jordan Journal of Biological Sciences
Issue
Vol. 5, Issue 2 (30 Jun. 2012), pp.135-139, 5 p.
Publisher
The Hashemite University Deanship of Academic Research and Graduate
Publication Date
2012-06-30
Country of Publication
Jordan
No. of Pages
5
Main Subjects
Topics
Abstract EN
Embryonic Stem (ES) cells have the ability to differentiate under in vitro conditions into cardiomyocytes.
A transgenic α-myosin heavy chain (α-MHC+) ES cell line was generated, exhibiting puromycin resistance and expressing enhanced green fluorescent protein (EGFP) under control of the α-MHC+ promoter.
A puromycin-resistant, EGFP-positive α-MHC+ cardiomyocyte population was isolated with over 92 % purity.
The cultivation of these cardiomyocytes, in macroporous gelatine microsphere beads in a spinner flask bioreactor has been studied.
After we specified the most suitable agitation conditions and the optimal timeframes of cultivation, the average number of cultivated cells per microsphere was optimised.
Our study shows that 80 % of microspheres were colonised by cardiomyocytes under optimal conditions.
The results of the scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) showed that the population of the beads was not limited to the microcarrier surface, but also invaded the inner surfaces of the microspheres.
The present findings demonstrate the successful culture of α-MHC+ cardiomyocytes in macroporous biodegradable microcarriers while maintaining the typical morphological and electrophysiological properties of cardiomyocytes.
These findings suggest significant improvement in survival of grafted cardiomyocytes, thus helping overcome current limitations of cell replacement approaches.
American Psychological Association (APA)
Akasha, Abd al-Rahman A.& Elouz, Abd al-Rahim A.. 2012. Attachment of embryonic stem cells-derived cardiomyocytes in cultispher-S microcarriers by using spinner flask. Jordan Journal of Biological Sciences،Vol. 5, no. 2, pp.135-139.
https://search.emarefa.net/detail/BIM-311191
Modern Language Association (MLA)
Akasha, Abd al-Rahman A.& Elouz, Abd al-Rahim A.. Attachment of embryonic stem cells-derived cardiomyocytes in cultispher-S microcarriers by using spinner flask. Jordan Journal of Biological Sciences Vol. 5, no. 2 (Jun. 2012), pp.135-139.
https://search.emarefa.net/detail/BIM-311191
American Medical Association (AMA)
Akasha, Abd al-Rahman A.& Elouz, Abd al-Rahim A.. Attachment of embryonic stem cells-derived cardiomyocytes in cultispher-S microcarriers by using spinner flask. Jordan Journal of Biological Sciences. 2012. Vol. 5, no. 2, pp.135-139.
https://search.emarefa.net/detail/BIM-311191
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 139
Record ID
BIM-311191