Genotypic analysis of E. coli strains isolated from patients with cystitis and pyelonephritis
Joint Authors
Japoni, A.
Alborzi, A.
Anvarinejad, M.
Farshad, Sh.
Ranjbar, R.
Giammanco, Giovanni M.
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 14, Issue 7 (31 Jul. 2012), pp.408-416, 9 p.
Publisher
Publication Date
2012-07-31
Country of Publication
United Arab Emirates
No. of Pages
9
Main Subjects
Topics
Abstract EN
Background : urinary tract infection is the most common health problem affecting millions of people each year, mainly caused by a large genetically heterogeneous group of Escherichia coli called uropathogenic E.
coli.
This study investigates the genotypic analysis of E.
coli strains isolated from patients with cystitis and pyelonephritis.
Methods : during 2008-2009, 90 E.
coli strains were analyzed, consisting of 48 isolates causing pyelonephritis in children and 42 isolates causing cystitis.
Having identified the strains by standard methods, they were subtyped by pulsed field gel electrophoresis (PFGE) and their corresponding patterns were compared using dendogram.
Results : Sixty five PFGE profiles were obtained from the genome of E.
coli strains by this genotyping method.
Thirty six and thirty three patterns were obtained for pyelonephritis and cystitis, respectively.
Most strains exhibited twelve and thirteen bands and the patterns with eight or nineteen bands had the lowest rate.
Genome sizes of the strains were between 1610-4170 kbp.
Conclusion : with due attention to these results, genetic patterns showed that the strains had different clonal ties and it could be suggested in some cases that the strains causing pyelonephritis or cystitis have common patterns and different diseases could be explained by different gene factors.
American Psychological Association (APA)
Anvarinejad, M.& Farshad, Sh.& Ranjbar, R.& Giammanco, Giovanni M.& Alborzi, A.& Japoni, A.. 2012. Genotypic analysis of E. coli strains isolated from patients with cystitis and pyelonephritis. Iranian Red Crescent Medical Journal،Vol. 14, no. 7, pp.408-416.
https://search.emarefa.net/detail/BIM-311673
Modern Language Association (MLA)
Anvarinejad, M.…[et al.]. Genotypic analysis of E. coli strains isolated from patients with cystitis and pyelonephritis. Iranian Red Crescent Medical Journal Vol. 14, no. 7 (Jul. 2012), pp.408-416.
https://search.emarefa.net/detail/BIM-311673
American Medical Association (AMA)
Anvarinejad, M.& Farshad, Sh.& Ranjbar, R.& Giammanco, Giovanni M.& Alborzi, A.& Japoni, A.. Genotypic analysis of E. coli strains isolated from patients with cystitis and pyelonephritis. Iranian Red Crescent Medical Journal. 2012. Vol. 14, no. 7, pp.408-416.
https://search.emarefa.net/detail/BIM-311673
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 414-416
Record ID
BIM-311673