The validity of immunocytochemical expression of cyclin D1 in fine needle aspiration cytology of breast carcinoma
Joint Authors
Source
Journal of the Egyptian National Cancer Institute
Issue
Vol. 24, Issue 3 (30 Sep. 2012), pp.145-150, 6 p.
Publisher
Cairo University National Cancer Institute
Publication Date
2012-09-30
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Topics
Abstract EN
Purpose : The aim of this work is to study the validity of cyclin D1 expression, a cell cycle regulatory protein, on (fine needle aspiration cytology) FNAC samples in patients with breast carcinoma using immunostaining technique.
Patient and methods : This is a study done on 70 patients with primary breast carcinoma, presented to Cytology Unit, Pathology Department, National Cancer Institute, Cairo University.
They underwent preoperative FNAC and diagnosed as breast carcinoma.
The cytologic and tissue section slides were subjected to cyclin D1 immunocytochemical staining.
Only the nuclear immunoreactivity for cyclin D1 was considered specific.
The rate of concordance, and discordance, and kappa value were calculated.
Relation between cytologic expression of cyclin D1 and different clinicopathologic parameters was evaluated.
Results : Cyclin D1 immunocytochemical expression was observed in 53 / 70 cases (75.7 %) in cytologic smears.
In histologic sections of the corresponding cases, cyclin D1was detected in 48 / 70 cases (68.6 %).
The concordance rate of cyclin D1 expression in the FNA and histologic sections was 87.1 % while the discordance rate was 12.9 %.
Kappa showed a value of 0.65.
A statistically significant relation was found between cyclin D1 immunocytochemical expression and hormonal status as well as nuclear grade.
Conclusion : Cyclin D1 immunocytochemical expression can be performed successfully on cytologic samples with a high concordance rate and agreement with histologic results.
This can help in determining tumor biology, and plan for patients’ treatment.
The marker showed a significant relation with hormone receptor status and nuclear grade.
American Psychological Association (APA)
Ezzat, Nuha& Hafiz, Nisrin. 2012. The validity of immunocytochemical expression of cyclin D1 in fine needle aspiration cytology of breast carcinoma. Journal of the Egyptian National Cancer Institute،Vol. 24, no. 3, pp.145-150.
https://search.emarefa.net/detail/BIM-312752
Modern Language Association (MLA)
Ezzat, Nuha& Hafiz, Nisrin. The validity of immunocytochemical expression of cyclin D1 in fine needle aspiration cytology of breast carcinoma. Journal of the Egyptian National Cancer Institute Vol. 24, no. 3 (Sep. 2012), pp.145-150.
https://search.emarefa.net/detail/BIM-312752
American Medical Association (AMA)
Ezzat, Nuha& Hafiz, Nisrin. The validity of immunocytochemical expression of cyclin D1 in fine needle aspiration cytology of breast carcinoma. Journal of the Egyptian National Cancer Institute. 2012. Vol. 24, no. 3, pp.145-150.
https://search.emarefa.net/detail/BIM-312752
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 149-150
Record ID
BIM-312752