Some rapid methods for oral treponema detection
Other Title(s)
بعض الطرق السريعة في تشخيص جراثيم تريبونيما الفم
Author
Source
Issue
Vol. 23, Issue 3 (30 Sep. 2012), pp.23-32, 10 p.
Publisher
University of Mosul College of Science
Publication Date
2012-09-30
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Topics
Abstract AR
تبحث الدراسة الحالية عن وجود أربعة أنواع من Treponema الفم باستخدام الطرائق الجزيئية السريعة.
تم الحصول على عينات من سائل تحت اللثة قبل و بعد اسبوع واحد من إجراء scaling و تم تحرير الحامض النووي من الخلايا البكتيرية لاستخدامه كقالبا في تفاعل PCR.
تم اعتماد بوادئ متخصصة لتتابع 16SrDNA لكل نوع Treponema.
أظهرت النتائج أن %89 من العينات كانت موجبة لوجود Treponema sp., %88 منها كانت موجبة لوجود أكثر من نوع واحد.
شخصت الأنواع T.
amylovorum, T.
medium, T.
socranskii, T.
vincentii بنسبة %72, %87, %76, %28 على التوالي و بنسبة أعلى عند عمق جيب 5< ملم.
تمكنت الطرائق الجزيئية من الكشف عن هذه الأنواع بعد إجراء scaling لكن بنسبة اقل من نسبتها قبل إجراء scaling.
Abstract EN
The present study investigates the presence of four oral Treponemal species using vrapid molecular methods.
Sub gingival fluid samples were obtained before and after one week of scaling and the nucleic acid was liberated from the bacterial cells to be used as a template for PCR.
Specific primers for each Treponemal species targeted to16SrDNA sequence was depended.89 % of samples were positive for Treponema sp., 88 % of them were positive to the presence of more than one type.
T.
amylovorum, T.
medium, T.socranskii, and T.
vincentii were detected in 72 %, 87 %, 76 %, and 28 % respectively with higher percentage at pocket depths > 5 mm.
Molecular method was able to detect Treponemal species even after scaling, however, in lower percentage than before it.
American Psychological Association (APA)
al-Hamdoni, Sumayyah A.. 2012. Some rapid methods for oral treponema detection. Rafidain Journal of Science،Vol. 23, no. 3, pp.23-32.
https://search.emarefa.net/detail/BIM-313132
Modern Language Association (MLA)
al-Hamdoni, Sumayyah A.. Some rapid methods for oral treponema detection. Rafidain Journal of Science Vol. 23, no. 3 (Sep. 2012), pp.23-32.
https://search.emarefa.net/detail/BIM-313132
American Medical Association (AMA)
al-Hamdoni, Sumayyah A.. Some rapid methods for oral treponema detection. Rafidain Journal of Science. 2012. Vol. 23, no. 3, pp.23-32.
https://search.emarefa.net/detail/BIM-313132
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 31-32
Record ID
BIM-313132