Cytogenetic toxicity effects of local purslane (portulaca oleracea)‎ leaf crude extracts on normal and cancer cell lines in vitro

Other Title(s)

التأثيرات السمية لمستخلص أوراق نبات الرجلة المحلي (Portulaca oleracea)‎ على الوراثة الخلوية للخطوط الخلوية السرطانية و الطبيعية

Dissertant

Shaykhah, Zakariyya Ahmad

Thesis advisor

Hidayat, Hazha Jamal

Comitee Members

Ismail, Nazim J.
Wahid, Intisar Numman
Faraj, Kawis A.

University

Salahaddin University-Hawler

Faculty

College of Science

Department

Biology Department

University Country

Iraq

Degree

Master

Degree Date

2012

English Abstract

The present study was carried out to evaluate the cytogenetic toxicity effect of aqueous and ethanol crude extracts of the leaf of Portulaca oleraceal L.against two malignant cell lines which were Murine mammary adenocarcinoma (AMN3), human Rhabdomysarcomas (RD) and one normal cell line which were kidney epithelium of African green monkey (Vero).

Also the study included the angioproliferative effects of aqueous and ethanol crude extracts of P.oleracea leaf on the mitotic index (cell division) of Vero, AMN3 and RD cell lines in vitro.

The aqueous crude extract was found to be more effective ant proliferate agent than the ethanol crude extract.

Both extracts exhibited time-dependent cytotoxic effects against AMN3 and RD cancer cell lines.

In which AMN3 was more sensitive to the extracts than RD.

All concentrations of aqueous extract were affected AMN3 cell line at 72hrs.

Of exposure, but for ethanol extract the last dose (0.01μg / ml) was not effective.

While the Vero normal cell line showed resistance toward all concentrations of both extracts except the first dose (10000 μg / ml), at all time of exposure (24, 48 and 72)hrs.

Also the angioproliferative effects of aqueous and ethanol crude extracts of Portulaca oleraceal leaf were tested for mitotic index for Vero, AMN3 and RD cell lines in vitro after being treated to the extracts at (10000 and 1000)μg / ml for three times of exposure (24, 48 and 72)hrs.

It is revealed that the reduction in mitotic index in both cancer cell lines (AMN3 and RD) was found especially at 72 hrs.

Of exposure, in which both crude extracts had the same effects on cell division, and showed time-dependent inhibitory effects.

But the reduction of mitotic index in Vero normal cell line has been less noted, especially at 24 and 48 hrs.

Of exposure.

But at the 72hrs of exposure showed some reduction in cell divisions when compared to the control

Main Subjects

Biology

Topics

• Cancer cell

No. of Pages

100

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-314603