The effect of Tamarix ramosissima and Cinnamomum zeylanicum extracts on the growth of Candida spp. isolated from the saliva of type II diabetes mellitus patients

Abstract EN

Background : Plants have an almost limitless ability to synthesize aromatic substances, most of which are phenols or their oxygen-substituted derivatives.

These secondary metabolites could be utilized for benefit of mankind by studying their medicinal properties The study was performed for isolation and identification of the oral Candida species in patients with type II Diabetes mellitus and healthy subjects (control group) and testing the effect of Tamarix ramosissima (T.

ramosissima)and Cinnamomum zeylanicum (Ci.

Zeylanicum)extract on the isolated species. Materials and Methods : Fasting stimulated saliva were collected by spitting method for each subject.

Candida species were isolated, purified and identified according to morphological characteristic on chromogenic agar (CHROMagar Candida) and biochemical test on API Candida.

Also the study included the effect of T.

ramosissima and Ci.

zeylanicum extracts on the isolated species using agar well diffusion method.

Aqueous, alcoholic, ethylacetate and aqueous acetone extracts were prepared in different concentrations T.

ramosissima and aqueous, alcoholic extract from Ci.zeylanicum.

The activity of the extract was determined by measuring the diameter of inhibition zone. Results : The results showed that Candida species was recovered from the oral cavity of 76 % of the diabetic group, in contrast to 8.58 % of the control group.

Candida albicans (C.

albicans) was the most frequently isolated species in both groups it reach to 33 isolates (66%) (in diabetic group fallowed by C.

krusei two isolates (4 %), C.

glabrata one isolate (2 %), C.tropicalis one isolate (2 %) and C.

lusitaniae one isolate (2 %) and three isolates (8.58 %) of C.

albicans in the control group.

There was highly significant p<0.01 correlation between glycemic control and age, significant P < 0.05 correlation between females and diabetes with oral carriage of Candida species, also results showed highly significant p < 0.01 antifungal effect of Tamarix ramosissima aqueous acetone extract on all Candida species by using different concentrations there ; were diameters of inhibition zones found to increase with increasing the concentration of the extracts.

While aqueous, alcoholic and ethylacetate extract of Tamarix ramosissima did not show any effect on Candida species.

Highly significance p < 0.01 was shown in aqueous, alcoholic extracts of Cinnamomum zeylanicum on all Candida species ; the diameter of inhibition increase with increasing the concentration. Conclosion : Diabetic patients had a higher oral candidal carriage rate in comparison to non- diabetic.

C.

albicans was the predominant isolate, and a variety of other Candidal species.

Oral candidal colonization was highly significantly associated with fasting blood sugar.

Tamarix.

ramosissima leaves (aqueous acetone) extract have antifungal effect against Candida while (ethylacetate, aqueous and alcoholic) extract don’t have antifungal effectagainst Candida isolates from the oral cavity of Diabetes mellitus type II.

Cinnamomum.

zeylanicum bark (aqueous and alcoholic) extract have antifungal effect against Candida isolates from oral cavity of Diabetes mellitus type II.