Polymerase chain reaction amplification of the ITS-1 region and sequencing of the amplified products of most pathogenic Aspergillus spp. (A.fumigatus, A.flavus & A.niger)‎

Abstract EN

The internal transcribed spacer region (ITS) contain variable elements that allow sequence-based identification of genus Aspergillus.

In this study, a PCR products of 521 b.p.

were successfully amplified from 6 Aspergillus spp.

isolated from granulomatous lesions of lungs of chickens which were identified by traditional methods to A.fumigatus (2), A.flavus (1) , A.niger (2) and A.terreus (1) with pan Aspergillus primer for amplification of the ITS1 region.

Three PCR products of A.fumigatus, A.flavus and A.niger were sequenced and the results were aligned with the sequences published on the gene bank.

The first sample gave alignment with A.fumigatus, 2nd sample gave alignment with A.flavus and A.oryzae and the 3rd sample gave alignment with A.niger var ficuum.

The phyelogenetic analysis revealed that the degree of similarity between the isolated strains of A.niger, A.flavus and A.fumigatus was less than 50 % so, we could conclude that the importance of ITS-1 region sequence to distinguish between the different Aspergillus species.