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Estimation activity and partial purification of leucine amino peptidase (lap) in patients wiith diabetic nephropathy
Other Title(s)
تعيين فعالية إنزيم الليوسين امينوببتايد في المرضى المصابين بالسكر الكلوي و تنقيتة جزئيا
Author
Source
Issue
Vol. 9, Issue 4 (31 Dec. 2012), pp.689-694, 6 p.
Publisher
University of Baghdad College of Science for Women
Publication Date
2012-12-31
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Topics
Abstract AR
تم قياس إنزيم الليوسين أمينو ببتايديز (LAP) في أمصال 50 مصاب بالفشل الكلوي السكري و 50 شخص سليم (مجموعة قياسية).
هدفت الدراسة الحالية بيان تأثير الفشل الكلوي السكري على مستوى أنزيم (LAP) و تنقية جزئية (LAP) في أمصال المرضى المصابين بالفشل الكلوي السكري.
لوحظ وجود زيادة إحصائية مقبولة في مستوى (LAP) (P<0.001) للمرضى مقارنة بالأصحاء.
نقي (LAP) من أمصال المرضى المصابين بالفشل الكلوي السكري باستخدام أكياس الفرز الغشائي, و كروموتوغرافيا الترشيح بالهلام (Sephadex G-25).
(1.37) عدد مرات تنقية أنزيم (LAP) من أمصال المرضى المصابين بالفشل الكلوي السكري باستخدام أكياس الفرز الغشائي, أعطي أنزيم (LAP) قمة واحدة باستخدام كروموتوغرافيا الترشيح بالهلام (Sephadex G-25) بأبعاد (20 x 15 cm) و بعدد مرات تنقية (8.33).
Abstract EN
Leucine aminopepotidase (LAP) [EC : 3.4.11.1] activity has been assayed in (50) serum samples of patients with diabeties nephropathy D.N (non-insulin dependent diabetic (NIDD), and (50)serum sample of healthy individuals without any clinically detectable diseases have been as control group.
The aim of this study is to measure leucine aminopeptidase activity and partially purifying the enzyme from sera of patients with diabetes nephropathy The results of this study revealed that Leucine aminopeptidase (LAP) activity of nephropathy patient’s serum shows a high signifiacant increase (p < 0.001) compared to that of the healthy subjects.
LAP was purified from the serum of patients with diabetes nephropathy by dialysis and gel filtration (Sephadex G-25) (fine) (20 × 1.5 cm) .A (1.37) fold purification of serum LAP from patients serum with diabetic nephropathy was achieved by using dialysis and this enzyme showed single grade increased to (8.33) fold by using gel filtration Abbreviation : Leucine aminopeptidase=LAP, Diabetes Nephropathy = D.N, Non- Insulin dependent diabetic = NIDD.
American Psychological Association (APA)
Hamid, Arij Shawkat. 2012. Estimation activity and partial purification of leucine amino peptidase (lap) in patients wiith diabetic nephropathy. Baghdad Science Journal،Vol. 9, no. 4, pp.689-694.
https://search.emarefa.net/detail/BIM-316700
Modern Language Association (MLA)
Hamid, Arij Shawkat. Estimation activity and partial purification of leucine amino peptidase (lap) in patients wiith diabetic nephropathy. Baghdad Science Journal Vol. 9, no. 4 (2012), pp.689-694.
https://search.emarefa.net/detail/BIM-316700
American Medical Association (AMA)
Hamid, Arij Shawkat. Estimation activity and partial purification of leucine amino peptidase (lap) in patients wiith diabetic nephropathy. Baghdad Science Journal. 2012. Vol. 9, no. 4, pp.689-694.
https://search.emarefa.net/detail/BIM-316700
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 692-693
Record ID
BIM-316700