The relation of TEL-AML1 fusion genes, mutant P53 gene and B19 parvovirus infection in newly diagnosed pediatric acute lymphoblastic leukemia, in Basrah

Other Title(s)

مدى علاقة الاندماج الجيني TEL-AML1 بالطفرات الوراثية لجين P53 و أخماج فايروس بارفو ب 19 لدى الأطفال المصابين بمرض ابيضاض الدم اللمفاوي الحاد في البصرة

Dissertant

al-Musawi, Wijdan Nizar Ibrahim

University

University of Basrah

Faculty

Medicine College

Department

Department of Microbiology

University Country

Iraq

Degree

Ph.D.

Degree Date

2011

English Abstract

TEL-AML1 fusion gene, resulting from 12; 21 chromosomal translocation, is believed to be the most common molecular genetic abnormality in childhood acute lymphoblastic leukemia (ALL).

This study has been conducted to investigate the frequency of this fusion gene in children suffering from ALL attending the Oncology Unit in Al-Basrah Hospital for Pediatrics and Gynecology during the period of May 2009- April 2010, and to point out the different laboratory features associated with this anomaly, as well as the status of p53 protein and their relation to Human Parvovirus B19 infection. A total of 100 blood sample were collected (from 40 newly diagnosed acute lymphoblastic leukemia ALL) and 60 from healthy children as a control group (Case –Control Study).The controls were approximately matched the cases regarding age and sex. Ribonucleic acid (RNA) had been extracted from the fresh blood samples in order to detect TEL-AML1 fusion genes using reverse transcriptase polymerase chain reaction (RT-PCR). Anti-p53 antibody and Human Parvovirus B19 (HPV B19) IgG was demonstrated by using (Enzyme Linked Immunosorbent Assay ELISA). Of the newly diagnosed ALL cases, 11 were positive for TEL-AML1 fusion gene (27.5 %), as well as 3 out of the 60 control group (5 %). All positive cases to TEL-AML1 showed an age peak between 3 and 6 years, it tends to occur more in the female than male.

TEL-AML1positive cases that classified as standard risk group accounted for 72.7 % while 27.3 % were high risk group (P< 0.05). The overall proportion of anti-P53 antibody was 20% in the leukemic patient, whereas none of the healthy group showed positive result this difference was statistically significance (P < 0.05).

According to risk groups 75% of the Anti-P53 positive cases were classified as high risk patients.

There was significant difference (P< 0.05) in the frequency of anti-P53 antibody between standard and high risk group ALL patients, 40 % of the high risk group had anti-P53 antibody as compared to 8 % in the standard risk patients.

None of the standard risk patient with positive TEL-AML1 translocation had anti-P53 antibody, while 33.3 % of TEL-AML1positive high risk patient were positive to anti-P53 antibody and it's statistically not significance (P > 0.05). The HPV B19 IgG antibody had been detected by ELISA test in order to shed light on the role of B19 infection in induction or progression of leukemogenesis.

Higher proportion of Human Parvovirus B19 positive cases was found among leukemic patient (47.5 %) as compared to 20 % in the control group (P < 0.05).There was significant association between TEL-AML1 translocation and Human Parvovirus B19 infection : we found that 71.4 % of TEL-AML1 positive cases in the study population had Human Parvovirus B19 IgG.

On the other hand there was no association between Human Parvovirus B19 infection and P53 gene mutation in our studied cases> In conclusion, TEL-AML1 fusion gene identifies a subset of pediatric acute lymphoblastic leukemia associated with a number of clinical and laboratory markers of good prognosis and should thus be included in routine molecular workup of acute leukemia to confirm its impact on clinical outcome and to design suitable therapeutic regimens.

Human Parvovirus B19 infection is common and highly distributed infection in our locality, with more common prevalence among leukemic patients.

The study revealed a significant association between Human Parvovirus B19 infection and TEL-AML1 fusion genes in ALL patients.

Main Subjects

Medicine

Topics

• Children
• Leukemia
• Iraq
• Basra
• Genetic aspects

No. of Pages

137

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-316900