Some immunogenetic aspects for diagnosis of hepatitis B virus in Basrah province-Iraq

Other Title(s)

بعض النواحي المناعية الوراثية لتشخيص التهاب الكبد الفيروسي البائي في محافظة البصرة-العراق

Dissertant

al-Hammudi, Haydar Abd al-Husayn Muklif

Thesis advisor

Isa, Awatif Hamid
Hammadi, Sad Shahin

Comitee Members

al-Mamuri, Abd al-Nabi J.
Jafar, Adnan M.
al-Hilali, Hammadi Abtan
al-Edani, Thar A.
Abd Allah, Basimah Ahmad

University

University of Basrah

Faculty

Science College

Department

Department of Biology

University Country

Iraq

Degree

Ph.D.

Degree Date

2011

English Abstract

The study was carried out to investigate some immunogenetic aspects in Basrah patients with hepatitis B virus infection.

The study included 205 individuals of both sexes, comprising 155 individuals as the patients group, consisting of 122 males, 33 females with age ranged 3-80 years and 50 individuals randomly selected as control groups consisting of 29 males, 21 females with age ranged 20-50 years. The total markers of HBV (HBsAg, anti-HBs, HBeAg, anti-HBe, Total anti-HBc and IgM Anti-HBc) were detected by application of ELISA technique.

The results showed a positive sera of 151 patients, thus a total of 155 individuals divided into usual markers (78.72 %) and unusual markers (18.7 %) in addition to (2.58 %) of individual groups of vaccinated and non-infected.

Usual markers of patients were divided into eight studied groups including : Incubation ( 2.58 %),Active acute (3.87 %) , Acute (13.55 %) , Active chronic ( 1.29 %) , chronic ( 5.17 %) , Inactive HBsAg Carrier (44.52 %),HBsAg-negative patients ( 5.8 %) and liver cancer (1.94 %) groups.

According to annual statistical the prevalence of HBV infection which was observed in public health department and blood bank center at period 2005-2010 were 1.36 % and 0.27 %, respectively. The present study showed that 78.14 % of the males in both markers more susceptibility to the infection with highly significant differences (P < 0.001) than females (21.86 %).

From these, Married patients (61.59%) showed significantly elevated (P < 0.05) than single patients (38.41 %).

Also (44.37 %) uneducated patients, (97.35 %) non-vaccinated patients and (94.7 %) non- treated patients were more susceptible to the infection with highly significant differences (P < 0.001) in usual markers and unusual markers, respectively. Furthermore, the age ranges of the infected patients varied between 3 and 80 years .The age period between (20-29) and (30-39) were significantly prone to the infection (P < 0.05).

In contrast, the age period (10-19) years was significantly decreased to the infection (P < 0.05).

Interestingly, both markers of patients showed no significant differences among different symptoms.

Big portion (33 %) of both markers of patients acquired HBV infection through unknown routes of infection, particularly in Male patients (P < 0.001).

Blood transfusion and contacting with HBV Patients form the main probable routes of transmission (25 %) ; significant differences (P < 0.001). Analysis of HLA complex was carried out for 50 patients with chronic HBV infection and 50 healthy control persons to have a clearer integrative idea on the impact of HLA (class II ,HLA- DRB1 plus ) on the susceptibility and resistance to HBV infection.

Alleles DRB1 * 3 (P = 0.001) sub-allele DRB1 * 030101 (P = 0.007) and DRB1*11 (P = 0.014) were might be responsible for susceptibility.

Alleles DRB1 * 01(P = 0.006), sub-allele DRB1* 070101(P = 0.015) and DRB1* 13 (P = 0.013) were might be responsible for resistance. On the other hand this study determinated the genotype of HBV in Basrah patients for the first time.

The genotype D was the only detected type found in all patients. sFas levels (Mean ± SD ) showed highly significant differences (P< 0.001) among studied groups.

Chronic (2.34 ± 0.69), Inactive HBsAg carrier (1.08 ± 0.36) and liver cancer (2.69 ± 0.35) groups had elevated mean values in comparison with healthy control (0.64 ± 0.12).

Also sFasL levels (Mean ± SD) showed significantly elevated (P=0.003) in chronic (0.1 ± 0.02) and significant differences (P = 0.02) in Inactive HBsAg carrier (0.08 ± 0.03) in comparison with healthy control (0.06 ± 0.01). Liver function tests were more significantly elevated (P < 0.001 or 0.05) in liver cancer, unusual markers and chronic groups than other groups.

Main Subjects

Medicine

Topics

• Iraq
• Basra
• Diagnosis
• Hepatitis B

No. of Pages

124

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-317060