In vitro effects of alcohol garlic extraction, formalin and glucose on protoscolices of echinococcus granulosus

Abstract EN

This study to evaluate the protoscolicidal effects of various concentration of alcohol garlic extraction ,formalin and glucose.

Liver and lung protoscolices of sheep were exposed to 100ul, 200 ul, 500 ul and 1000 ul of alcohol garlic extraction ; 100ul ,500 ul and 1000 ul of formalin ; 10 %, 20 %, 30 % and 50 % of glucose were used as the positive control ,while physiological saline was used as negative control.

Viability of protoscolices was determined using 1 % of aqueous eosin stain method.

The percentage (100 %) of mean of dead protoscolices after 1 hr, 2.5 hr, 3 hr and 5 hr of 1000 ul, 500 ul, 200 ul and 100 ul of alcohol garlic extraction respectively.

The statistical analysis (T- test) showed that was significant differences between the means protoscolicidal effects of alcohol garlic extraction and physiological saline (P < 0.01). The percentage average of dead protoscolices (100%) in (10, 15, 20) min after exposure with (1000, 500 and 100) ul of formalin respectively.

The statistical analysis showed that there was significant difference between the protoscolicidal effect of formalin and physiological saline (P < 001). The percentage average of dead protoscolices (100 %) was noticed with 50 % of glucose after exposure 10 min and3 hr, 4 hr after treated with 30 % and 20 % of glucose respectively.

Significant difference was observed between the means protosclicidal effect of glucose and physiological saline ( P < 00.1).