Staining technique for helminth parasites by use red beet (Beta vulgaris L.)‎ extract

Abstract EN

Experimentally red beet (Beta vulgaris L.) extract had been used to stain different types of parasites Lecithochirium acutum (Trematoda), Taenia spp.Dipylidium caninum and cysticercus cyst for Taenia spp.

(Cestoda), Toxascaris leonina and Toxocara canis (Nematoda) and Neoechinorhynchus iraqensis (Acanthocephala).

Stained helminthes were acquired a good pigmentation with distinction their internal structure, fish's Trematoda Lecithochirium acutum appear well –defined of its internal structure, the oral and ventral sucker, cirrus sac, uterus, testes, ovary and vitallaria were appeared pink in color.

The whole of mature and gravid segments of Taenia spp.

were acquired the yellow to brown colour in both cirrus sac and vas deference besides differentiation of eggs in gravid segment, Scolex of Taenia spp.

get brown in color.

in addition segment of Dipylidiun caninum acquired yellow colour.

The cysticercus cyst was taken pink in color with distinguish of its hooks, while both Toxascaris leonina, Toxocara canis and Neoechinorhynchus iraqensis were appear pink to red in colour .