Immunological and molecular study to some chicken allergens in allergic patients

Other Title(s)

دراسة مناعية و جزيئية لبعض مستأرجات الدجاج في مرضى الحساسية

Dissertant

Jasim, Raghad Malih

Thesis advisor

Abd Allah, Fawziyah Ali
al-Rawdan, Adnan Musa

Comitee Members

Humim, Sad S.
Suhain, Abd Allah A.
Abbas, Basil Abd al-Zahrah

University

University of Basrah

Faculty

Veterinary College

Department

Department of Microbiology

University Country

Iraq

Degree

Master

Degree Date

2012

English Abstract

The partially purified chicken dropping and feather allergen extracts were prepared by extraction and dialysis. Direct, indirect and inhibition ELISA tests were used to detect total IgE, specific IgE and allergen cross-reactivity respectively.

Beside that polymerase chain reaction technique was performed to detect genetic relationship between allergy and HLA-DQB1*02 and HLA-DQB1 * 0302. The distribution of chicken dropping and feather allergen was determined by specific IgE based ELISA testing of 190 symptomatic and asymptomatic allergic patients attending the center of asthma and allergic disease in Basra governorate during September 2011.

The result of this test revealed that the overall rate of chicken allergens distribution was 94.7 % and the difference in the rate of sensitivity to allergen as a single or association of both allergens was non significant (P > 0.05) in different sex and age of studied individuals.

The overall rate of sensitivity to both allergens (73.68 %) was higher than that of single allergen (21.05 %).

On the other hand the both allergens sensitive patients males and second age group (> 45-75) patients showed higher rate of seropositivity (80.35 % and 80 % respectively) while the single allergen (dropping) sensitive patients females and those patients in the first age group (< 15-45) showed the higher rate of seropositivity (23.88 % and 22.67 % respectively). According to the distribution of feather, dropping allergens based ELISA positive results in symptomatic and asymptomatic studied individuals, the higher rate of seropositivity (95.8 %) was observed against chicken dropping.

In contrast the asymptomatic individuals showed higher rate of seropositivity (75 %) against chicken feather allergen.

The difference between symptomatic and asymptomatic seropositivity rates was significant (P < 0.05). The total IgE based ELISA results revealed significant difference (P < 0.05) in the rate of three types of allergy (questionable, probable and not probable) and the higher rate of allergic patients (47.37 %) showed allergy questionable while (35.26 %) of them were within very probable allergy and (17.37 %) was the rate of patients within not probable allergy.

In concern to symptoms of allergy and the concentration of total IgE there was significant difference (P < 0.05) between symptomatic and asymptomatic patients in the concentration of total IgE.

The higher rate (95.5 %) of very probable allergy was observed in symptomatic patients.

Also the significant difference (P < 0.05) was observed in the seropositivity against the tested allergens in the patients of the three types of allergy (questionable, probable and not probable).

The patients complain very probable allergy showed the higher rate of seropositivity against both dropping and feather allergens (98.5 % and 76.1 % respectively).

The positive feather and dropping allergens based ELISA results (mean ± SD of optical density value) of the allergic patients sera depending on the sex and age revealed significant difference (P < 0.05) in the mean ± SD value in concern to sex but the significant effect of age observed only in case of chicken dropping allergen sensitive patients. The ELISA inhibition test revealed complete inhibition 100 % between chicken feather and dropping allergens and the extract of these materials independently accounted for total allergenic activity.

The PCR analysis results revealed that the overall frequency of HLA-DQB1 * 0302 and HLA-DQB1 * 02 as a single alleles was observed in (10 %) and (7 %) of allergic patients respectively and the association of these alleles was observed in (62 %) of patients. According to age and sex of patients, the present result revealed the higher rate of HLA-DQB1 * 0302 and HLA-DQB1 * 02 as a single allele was observed in first age group (10.67 % and 9.33 % respectively).

While both alleles appeared in higher rate (64 %) of the patients of second age group.

In concern to sex the higher rate 18.2 % and 9.1 % of HLA-DQB1 * 0302 and HLA-DQB1 * 02 respectively appeared in females while 77.78 % of males showed both associated alleles.

In concern to allergy symptoms, the symptomatic patients appeared in higher rate of PCR positivity (12.5% and 8.75 %) with HLA-DQB1 * 0302 and HLA-DQB1 * 02 respectively, while asymptomatic patients showed the higher rate (65 %) of PCR positivity to the associated alleles. Depending on the relationship between the total IgE based ELISA and PCR results, 86.49%, 78.05% and 68.18 % of allergic patients with the three types of allergy (questionable, probable and not probable allergy respectively) showed positive PCR results.

The first allele (HLA-DQB1 * 02) appeared in higher rate (9.7 %) of allergic patients with allergy questionable while the second allele (HLA-DQB1 * 0302) appeared in higher rate of PCR positivity (13.64 %) in the patients who complain allergy not probable.

The association of both alleles was observed in higher rate of PCR positivity (86.49 %) in patients with very probable allergy.

Main Subjects

Zoology

Topics

• Diseases
• Allergy
• Chickens

No. of Pages

105

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-317235