Effect of L-Arginine on spermatogenesis of the diabetic rat

Abstract EN

L-Arginine has hypoglycemic and antioxidant effect in Alloxan diabetic animals and reduce effect of diabetes complication on spermatogenesis.

antioxidant have essential effect on spermatogenesis, L-Arginine has antioxidant and hypoglycemic effect.

Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Sixteen mature male rats aged 10 weeks ,were randomly divided into four equal groups as follows 1st diabetic group (DG) that received 150 mg/kg (IP) Alloxan single dose ;2nd group diabetic group treated with (L-Arginine-Hcl)(DAG) received 150mg/kg (IP) Alloxan as single dose plus L-Arginine-Hcl 200mg/kg(IP)-per day, 3ed group treated with(L-Arginine-Hcl)(AG) received 200mg/kg-(IP) perday ,and 4th were control group(CG) non treated .In 60 day the blood samples collected from heart to make serological parameters (glucose level) and testes removed to observe their Histopathological. Serum Glucose concentration showed a significant increase (p<0.05) in diabetic group (DG) compared with other groups while diabetic treatment group that has received L-Arginine 200 mg/kg(IP) (DAG) showed a significant (p<0.05) decrease compared with (DG).

The testes statistical analysis showed significant decrease (p<0.05) in testes weight in diabetic group compared with other groups. No histopathological changes was observed in both control and Arginine groups (CG &AG),meanwhile the histopathological change in testes of diabetic group (DG) showed Epidedymal tubules showed completely empty, The DAG showed Epidedymal tubules of DAG group showed little spermatozoa. Since in our study 200 mg/kg (IP) L-Arginine have significantly Preventive effect on serum glucose level and spermato-genesis, so it seems that using it can be effective for treatment in Diabetic Rat.