Detection of rfbO157 and fliCH7 genes in Escherichia coli isolated from human and sheep in Basrah province
Other Title(s)
الكشف عن جينات rfbO157 و flicH7 في بكتيريا الأشريشيا القولونية المعزولة من البشر و الأغنام في محافظة البصرة
Dissertant
Thesis advisor
Isa, Awatif Hamid
Khidr, Muhammad Hasan
Comitee Members
Abd al-Wadud, Isra
Khudayr, Bassam Yasin
al-Hilali, Hammadi Abtan
University
University of Basrah
Faculty
Veterinary College
Department
Department of Microbiology
University Country
Iraq
Degree
Master
Degree Date
2010
English Abstract
This study aimed to detect Escherichia coli O157 : H7 presence in samples from diarrhea patients, sheep, milk, and sheep drinking water and characterize it by selective media, biochemical tests, latex agglutination test, and PCR technique. During the period from November 2008 to May 2009, a total of 340 samples were collected (125 diarrhea patients feces, 125 healthy sheep feces, 45 milk, and 45 sheep drinking water samples).
All samples were screened for the presence of non-sorbitol fermenting colonies (NSF) on sorbitol MacConkey agar supplemented with Cefixime and potassium tellurite (CT-SMAC).
A total of 340 samples, 58 (46.4 %) from diarrhea patients, 98 (78.4 %) from sheep, 19 (42.2 %) from milk, and 17 (37.7 %) from water were non-sorbitol fermenting.
E.
coli isolates were serotyped as E.
coli O157:H7 by latex agglutination test, 3 (5.7 %) isolates of diarrhea patients, 7 (7.3 %) isolates of sheep, 2 (11.1%) isolates of milk, and none of water samples were positive to O157 : H7 latex test.
All the latex agglutination positive isolates were positive to PCR test except one isolate from sheep.
All the PCR positive isolates were positive to enterohemolysin production test on sheep blood agar.
All the identified isolates were found resistant to at least 3 antibiotics. The estimation of E.
coli O157 : H7 distribution in diarrhea patients samples during the period of study revealed that highest rate of isolation was observed in April (7.6 %) followed by December (4.7 %) while in sheep the highest rate was observed in March (10 %) followed by April (8.6 %).
Main Subjects
Topics
No. of Pages
93
Table of Contents
Table of contents.
Abstract.
Chapter One : introduction.
Chapter Two : review of literatures.
Chapter Three : materials and methods.
Chapter Four : results.
Chapter Five : discussion.
Conclusion and recommendation.
References.
American Psychological Association (APA)
al-Hasnawi, Faruq Latif Jasim. (2010). Detection of rfbO157 and fliCH7 genes in Escherichia coli isolated from human and sheep in Basrah province. (Master's theses Theses and Dissertations Master). University of Basrah, Iraq
https://search.emarefa.net/detail/BIM-317327
Modern Language Association (MLA)
al-Hasnawi, Faruq Latif Jasim. Detection of rfbO157 and fliCH7 genes in Escherichia coli isolated from human and sheep in Basrah province. (Master's theses Theses and Dissertations Master). University of Basrah. (2010).
https://search.emarefa.net/detail/BIM-317327
American Medical Association (AMA)
al-Hasnawi, Faruq Latif Jasim. (2010). Detection of rfbO157 and fliCH7 genes in Escherichia coli isolated from human and sheep in Basrah province. (Master's theses Theses and Dissertations Master). University of Basrah, Iraq
https://search.emarefa.net/detail/BIM-317327
Language
English
Data Type
Arab Theses
Record ID
BIM-317327
