Humoral immune response of Salmonella typhimurium and Salmonella enteritidis sonicated antigens in rabbits
Other Title(s)
الاستجابة المناعية الخلطية للمستضدات المكسرة لجرثومتي S. typhimurim و S. enteritidis في الأرانب
Joint Authors
al-Samirrai, Ikram Abbas Abbud
al-Shawi, Amal Majid Ali
al-Tai, Hala Rashid Said
Source
Iraqi Journal of Veterinary Medicine
Publisher
University of Baghdad College of Veterinary Medicine
Publication Date
2012-09-30
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Topics
Arabic Abstract
عزلت جرومتي S.typhimurium و S.
entertidis من الماعز ؛ و حضرمستضد السالمونيلا الكلي المكسر للنمط المصلى S.typhimurium و S.
entertidis و المستضد المركب (كلا النمطين) و تم تقييم فاعلية كل مستضد باستعمال فحص التلازن في الأنابيب و المقايسة المناعية الممتزة بالأنزيم (ELISA) باستعمال عشرين أرنب قسمت عشوائيا إلى أربعة مجاميع متساوية، منعت الأولى بمستضد WCS.
Ag.
S.
entertidis، الثانية بالمستضد WCS.
Ag.
S.
typhimurium، الثالثة WCS.
Ag compound و تركت الرابعة كمجموعة سيطرة أعطيت المحلول الملحي الوظيفي (2.pH7) تمثلت الاستجابة المناعية الخلطية بارتفاع مستوى الضدات و تركيز الكلوبيولين المناعي IgG في المجاميع الممنعة بعد اليوم 12، الشهر الأول، الثاني و الثالث من التمنيع و قد بلغ أعلى مستو لهما في الشهــــر الأول و كان معنويــــــــا (P < 0.01) عند التمنيع بالمستضد (449.65 ± 38.61 ng / ml,952 ± 20.85) WCS.
Ag compoundمقارنة مع المجاميع الممنعة الأخرى بالمستضدين WCS.Ag.S.
entertidis and WCS.
Ag.
S.typhimurium)).
أظهرت المجاميع الممنعة بالمستضد المركب بقاء زيادة مستوى تركيز الكلوبيولين المناعى IgG و الضدات خلال الشهر الثاني و الثالث،(218.90 ± 6.69 ng / ml, 528 ± 68.58 and 89.55 ± 2.63 ng, 280 ± 49.98) 528,m1/ng 69.6 90.218) على التوالي و كان معنويا (P < 0.01) عند التمنيع بالمستضدين الآخرين (WCS.
Ag.
S.
entertidis و WCS.
Ag.
S.
typhimurium) و كانت معدلات المجاميع الممنعة معنوية (P < 0.01) مقارنة بمجموعة السيطرة.
English Abstract
Salmonella typhimurium and salmonella enteritidis were isolated from infected goat and prepared antigens of whole cell sonicated antigen of S.typhimurium (WCS.
Ag.S.typhimurium), whole cell sonicated antigen of S.enteritidis (WCS.
Ag.S.
entertidis) and combination of whole cell sonicated antigen (Salmonella typhimurium and Salmonella enteritidis) (CWS.Ag).
Their efficacy was evaluated by using tube agglutination test and enzyme linked immune sorbent assay (ELISA).
Twenty rabbits were randomly divided into four groups; the 1st group was immunized by WCS.
Ag - Salmonella enteritidis, 2nd group immunized by (WCS Ags .typhimurium), 3rd group immunized by CWCS.Ag compound and 4th left as control group which injected by physiological buffer saline (pH 7.2).
The antibody titer was increased in after the day 12, first, second and third months of immunization by agglutination test.
IgG concentration was done by ELISA at the same time ; which were recorded a higher significant differences (p ≤ 0.01) at the first month in the group immunized by CWS Ag (449.65 ±38.6 1ng/ml IgG and 952 ± 20.85 antibodies titer ) compared with other immunized groups ( WCS–Ag–S.
enteritidis and WCS.Ag.S.typhimurium ).
Also, the IgG concentration and antibodies titer are still higher in the second and the third months in the immunized group by CWCS.Ag.
218.90 ± 6.69ng / ml, 528 ± 68.58 and 89.55± 2.63ng / ml, 280 ± 49.98 respectively with significant differences (p ˂0.01) compared with the immunized groups (WCS.Ag.S.
entertidis and WCS.
Ag.
S.typhimurium) and also, they are significant (p ≤ 0.01) when compared with the control group Research.
Data Type
Conference Papers
Record ID
BIM-317377
American Psychological Association (APA)
al-Samirrai, Ikram Abbas Abbud& al-Shawi, Amal Majid Ali& al-Tai, Hala Rashid Said. 2012-09-30. Humoral immune response of Salmonella typhimurium and Salmonella enteritidis sonicated antigens in rabbits. Veterinary scientific conference (11th : 2012 : Baghdad, Iraq). . Vol. 36, no. (s2) (2012), pp.84-88.Baghdad Iraq : University of Baghdad College of Veterinary Medicine.
https://search.emarefa.net/detail/BIM-317377
Modern Language Association (MLA)
al-Samirrai, Ikram Abbas Abbud…[et al.]. Humoral immune response of Salmonella typhimurium and Salmonella enteritidis sonicated antigens in rabbits. . Baghdad Iraq : University of Baghdad College of Veterinary Medicine. 2012-09-30.
https://search.emarefa.net/detail/BIM-317377
American Medical Association (AMA)
al-Samirrai, Ikram Abbas Abbud& al-Shawi, Amal Majid Ali& al-Tai, Hala Rashid Said. Humoral immune response of Salmonella typhimurium and Salmonella enteritidis sonicated antigens in rabbits. . Veterinary scientific conference (11th : 2012 : Baghdad, Iraq).
https://search.emarefa.net/detail/BIM-317377