The differences in bioactivity between crude and purified cholera toxin from local isolate of Vibrio cholera
Other Title(s)
الاختلافات في الفعالية الحيوية بين ذيفان الكوليرا الخام و النقي من بكتريا Vibrio cholerae المحلية
Joint Authors
al-Khafaji, Khulud Abd al-Ilah Muhammad
al-Thuwayni, Aminah Nimah
Source
Iraqi Journal of Veterinary Medicine
Publisher
University of Baghdad College of Veterinary Medicine
Publication Date
2012-09-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Arabic Abstract
في هذا البحث تمت تنقية ذيفان الكوليرا من العزلة المحلية المرضية و المسماة Vibrio cholera S و اعتمدت بضع خطوات لتنقية ذيفان الكوليرا شملت تركيز البرزتين و الاستخلاص المعاكس و الترشيح الهلامي.
أشارت الفعالية البايولوجية امتلاك الذيفان النقي و الخام لفعالية قاتلة للفئران البيضاء balb / C mice و سبب استعمال جرعة 2.5 مايكرورام من الذفان النقي موت للفئران مع ظهور علامات مثل تشنج العضلات و تسارع القلب بينما أدى الحقن تحت الجلد إلى حصول احمرار و أودمة للمناطق المحقونة.
أظهر الفحص النسيجي للكبد و الأمعاء و الغدة الأدرينالية و الطحال للفئران البيضاء أن أنسجة الكبد هي الأكثر تأثرا من الأنسجة الأخرى.
English Abstract
In this investigate Cholera toxin was purified from the local clinical isolate designated as V.
cholera S.
Few steps were employed for purification of CT including concentration of the protein, back extraction, and gel filtration.
The bioactivity of CT reflected that crude and purified toxin had lethal activity on Balb / c mice in which a dose of 2.5μg / ml of purified toxin caused mice death with symptoms such as muscle cramps and tachycardia while erythematous and induration was seen at intradermal I.
d injection of skin of Guinea pig.
Histopathological examination for liver, intestine, adrenal gland and spleen of white mice showed that liver tissue was more affected compared with spleen, intestine and adrenal gland tissues.
Data Type
Conference Papers
Record ID
BIM-317378
American Psychological Association (APA)
al-Thuwayni, Aminah Nimah& al-Khafaji, Khulud Abd al-Ilah Muhammad. 2012-09-30. The differences in bioactivity between crude and purified cholera toxin from local isolate of Vibrio cholera. Veterinary scientific conference (11th : 2012 : Baghdad, Iraq). . Vol. 36, no. (s2) (2012), pp.76-83.Baghdad Iraq : University of Baghdad College of Veterinary Medicine.
https://search.emarefa.net/detail/BIM-317378
Modern Language Association (MLA)
al-Thuwayni, Aminah Nimah& al-Khafaji, Khulud Abd al-Ilah Muhammad. The differences in bioactivity between crude and purified cholera toxin from local isolate of Vibrio cholera. . Baghdad Iraq : University of Baghdad College of Veterinary Medicine. 2012-09-30.
https://search.emarefa.net/detail/BIM-317378
American Medical Association (AMA)
al-Thuwayni, Aminah Nimah& al-Khafaji, Khulud Abd al-Ilah Muhammad. The differences in bioactivity between crude and purified cholera toxin from local isolate of Vibrio cholera. . Veterinary scientific conference (11th : 2012 : Baghdad, Iraq).
https://search.emarefa.net/detail/BIM-317378
