Studies on the effect of addition of korean red ginseng aqueous extracts on the fretilizing capacity of rabbit semen

Abstract EN

aqi One hundred sixty eight sexually mature Baladi Black (BB) rabbits (26 bucks aged 9 months and 142 monoparous does in two sequence fa parities) were used in the present study.

Semen was collected artificially, pooled and diluted with sodium citrate diluent.

Aqueous extracts of Korean red ginseng® were added to the diluted semen at different concentrations of 0 (control), 25, 50, and 75 ml, then the diluted \ semen was divided into two parts, the first part was stored at refrigeration temperature (4 - 6 °C) for up to 3 days while the second cuj part was incubated at 37 °C for up to 4 hrs.

Percentages of sperm ^n motility; alive and normal spermatozoa and acrosomal defects were mo recorded at the different stages of the preservation.

Sperm penetration into estrous cow cervical mucus was estimated during diluted semen ^ incubation.

Evaluation of the activities of glutamic-oxaloacetic transaminase (GOT); glutamic -pyruvic transaminase (GPT); acid & phosphatase (ACP) and alkaline phosphatase (ALP) enzymes in the BB ^ rabbit semen were measured during preservation.

One hundred and forty ^e two rabbit does in two consecutive parities were artificially inseminated 20c using either the diluted semen free or supplemented with 50 Korean • red ginseng extract/ 1 ml diluted semen (the level which showed the ^ ideal effects on semen quality) to estimate the fertility traits.

In both ^> parts of the supplemented diluted rabbit semen with Korean red -nfe ginseng® extract showed that there was significant improvement in: (P < 0.05) sperm motility, the percentages of alive spermatozoa, normal -^q spermatozoa and storageability, while the acrosomal defects percentages gj were significantly (P < 0.05) decreased.

Sperm penetration into estrous ^ cow cervical mucus were arranged significantly (P < 0.05) in descending ^ order as obtained by supplemented diluted semen with Korean red ^% ginseng® extract (50, 75, 25 nil ml.

and the control ones respectively).

q[z Also there were significant (P $ 0.05) decreases in the mean values of each of GOT, GPT, ACP and ALP either during preservation at refrigeration conditions or at incubation temperature.

Conception rate, ^% Kindling rate and litter size values were significantly (P < 0.05) better in ef ^ rabbit does inseminated artificially by using diluted semen supplemented with 50 ul/ ml aqueous extracts of Korean red ginseng® than those „ inseminated using extended semen free- Korean red ginseng extract.

In ^ conclusion, addition of Korean red ginseng® on diluted rabbit semen ^ might improve the semen quality during preservation at different ay temperatures and fertility traits of rabb:+ does inseminated artificially.

It was found the ideal level was the addition of korean ginseng aqueous extracts with 50 ul \ 1n diluted semen