Bacteriological, molecular and toxogenic activity aspects of campylobacter jejuni isolated from slaughtered cattle and sheep at Basrah city

Other Title(s)

الجوانب البكتريولوجية، الجزيئية و الفعالية الذيفانية لمنحنيات الصائم المعزولة من الأبقار و الأغنام المجزورة في البصرة

Dissertant

Radi, Lamya Yusuf

Thesis advisor

Khidr, Muhammad Hasan
Isa, Ali Abbud

University

University of Basrah

Faculty

Veterinary College

Department

Department of Microbiology

University Country

Iraq

Degree

Master

Degree Date

2012

English Abstract

The study was conducted from (December 2010 to June 2011).

During this study 330 samples were collected including bile samples (130 of sheep and 80 of cattle) and fecal samples (70 of cattle and 50 of sheep) from Basrah abattoir.

The samples were analyzed for checking the presence of Campylobacter jejuni by using selective media, biochemical, serological and biotyping tests.

Results of this study showed that the total rate of Campylobacter jejuni isolates was 13.3% of total samples.

However, isolation rate from bile was 17.6 % while from fecal samples was 5.8 % the differences in isolation rates was statistically significant (X2 = 7.24, P = 0.007, DF = 1).

The isolation rate from sheep was 14.5 % while from cattle was 11.5 % the differences in isolation rates was statistically significant (X2 = 0.46, P = 0.5, DF = 1).

The selective media were homemade including selective and enrichment broths (Preston enrichment broth with blood and Preston enrichment broth with hemin) and solid media (modified Charcoal Cefoperazoon Deoxycholat agar and modified Charcoal Cephalothin agar).

Morover, comparison between selective media was done to evaluate the best one.

Results of this study indicate that the best broth was Preston broth with hemin and the best solid media was modified Charcoal Cephalothin agar).

Eight isolates of C.

jejuni were investigated for the presence of genes encoded the cytolethal distending toxin (cdta, cdtb and cdtc genes) by using multiplex PCR assay.

The results revealed that cdtb was detected in 6 (75 %) of the isolates and cdtc of 2 (25 %).

The positive isolates of cdtb gene were assayed for production of enterotoxin in vivo by using rabbit ileal loop method.

The injected intestine by crude toxin revealed accumulation of fluid tenged with blood, moreover, histopathological study indicated presence of edema (in sub mucosa and muscular is layers) and villi were subjected to atrophy, congestion of blood vessels, hemorrhage and infiltration of inflammatory cells.

C.

jejuni isolates were subjected to antimicrobial susceptibility assays by using disc diffusion method.

The results showed that the percentage of isolates were sensitive to Gentamycine, Ciprofloxacin and Nitrofurantuone (95.5 %, 90.9 %, and 88.6 %) respectively, whereas, the percentage of susceptibility to Azithromycine, Erythromycine and Chloramphenicol were (68.2 %, 52.3 % and 34.1 %) respectively, on the other hand, all isolates were resistant to Tetracycline.

Main Subjects

Biology

Topics

• Cattle
• Iraq
• Basra
• Sheep

No. of Pages

99

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-317619