Synthesis of homoserine lactone from aspartic acid and induction of quorum sensing by E. coli isolates
Joint Authors
Kazim, Sawsan Hasan
Abd al-Latif, Lamis Abd al-Razzaq
Abd al-Razzaq, Muhammad Sabri
Source
al-Kufa University Journal for Biology
Issue
Vol. 4, Issue 2 (31 Dec. 2012)5 p.
Publisher
University of Kufa Faculty of Science Department of live Sciences
Publication Date
2012-12-31
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Abstract AR
في هذه الدراسة تم تصنيع Homoserine- L- lactone بواسطة بكتريا القولون المعوية من خلال إضافة حامض الأسبارتيك أسد إلى الوسط الزرعي بتركيز نهائي 1%.
و قد تم فحص إنتاج Homoserine- L- lactone في فترات مختلفة من الحضانة (بعد 2، 3، 4، 5، 6 و 24 ساعة).
حيث تم إضافة 0.01 % من KCN لمعرفة الوقت الذي يلاحظ فيه HSL في النمو البكتيري.
و قد وجد أن أفضل فترة لتراكم Homoserine- L- lactone هو بعد 4 ساعات من الحضانة التي وصل فيها Homoserine lactone إلى المستوى الأمثل.
و عندما تمت إضافة قطرة واحدة من النمو البكتيري من بكتريا القولون المعوية على الشريحة قد لوحظ تجمع للبكتريا دلالة على إنتاجها ل HSL.
Abstract EN
Homoserine L-lactone was synthesized by E.
coli through using aspartic acid which was added to the culture media at a final concentration 1 %.
Homoserine lactone production was checked at different intervals of incubation (after 2, 3, 4, 5, 6 and 24 hr.) where 0.01% of KCN is added to show the time of HSL appearance in the bacterial growth.
It was found that the best interval for accumulation of homoserine lactone was after 4 hours.
of incubation in which the homoserine lactone reached at optimal level.
When one drop of culture supernatant was added to fresh growth of E.
coli on slide, aggregation or quorum sensing was seen as indicator of HSL production.
American Psychological Association (APA)
Abd al-Latif, Lamis Abd al-Razzaq& Abd al-Razzaq, Muhammad Sabri& Kazim, Sawsan Hasan. 2012. Synthesis of homoserine lactone from aspartic acid and induction of quorum sensing by E. coli isolates. al-Kufa University Journal for Biology،Vol. 4, no. 2.
https://search.emarefa.net/detail/BIM-319001
Modern Language Association (MLA)
Abd al-Latif, Lamis Abd al-Razzaq…[et al.]. Synthesis of homoserine lactone from aspartic acid and induction of quorum sensing by E. coli isolates. al-Kufa University Journal for Biology Vol. 4, no. 2 (2012).
https://search.emarefa.net/detail/BIM-319001
American Medical Association (AMA)
Abd al-Latif, Lamis Abd al-Razzaq& Abd al-Razzaq, Muhammad Sabri& Kazim, Sawsan Hasan. Synthesis of homoserine lactone from aspartic acid and induction of quorum sensing by E. coli isolates. al-Kufa University Journal for Biology. 2012. Vol. 4, no. 2.
https://search.emarefa.net/detail/BIM-319001
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-319001