Isolation and identification of bactericide and miticide compound from schinus terebinthifolius leaves against the potato rot bacteria and spider mite

Abstract EN

The MeOH extracts of 26 plant species belonging to 18 plant families were examined against the two spotted spider mite Tetranychus urticae and the two pathogenic, brown rot bacterium Ralstonia solanacearum and soft rot bacterium Erwinia carotovora.

The results indicated that Schinus terebinthifolius was the most potent plant for controlling these pests as its methanol extract exerted highly activity against the two pathogenic bacteria in addition to moderate activity against the spider mite.

The MeOH extract of this plant was purified by using a combination of different chromatographic methods (column chromatography and TLC) to yield an active pure compound.

Based on spectroscopic methods (1H, 13C-NMR,UV and MS) the isolated compound was characterized as gallic acid methyl ester, which exerted a miticidal activity against the two spotted spider mite Tetranychus urticae (LC50 = 58 mg1-1) and antibacterial activity against Erwinia carotovora and Ralstonia solanacearum (MLC = 250 and 500µg / ml), respectively.