Identification of enterococcal species by polymerase chain reaction technique, with study of some immunological features
Other Title(s)
تشخيص المسبحيات المعوية الى مستوى النوع باستخدام تقنية Polymerase chain reaction مع دراسة بعض صفاتها المناعية
Joint Authors
al-Sadi, Muhammad A. K.
al-Sharrakh, Ala H.
Chabuck, Zaynab A.
Source
Issue
Vol. 9, Issue 4 (31 Dec. 2012), pp.824-832, 9 p.
Publisher
University of Babylon College of Medicine
Publication Date
2012-12-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract AR
تضمن البحث دراسة بكتريولوجية و جينية و بعض الصفات المناعية لجنس Enterococcous إذ تم الحصول على 7 عزلات من ضمن 200 عينة إدرار من المرضى.
لم يتم تشخيص العزلات إلى مستوى النوع باستخدام الفحوصات الكيموحيوية، على خلاف الفحص الدقيق و السريع لعزلات Enterococcous spp باستخدام Polymerase chain reaction (PCR)مع البوادئ المتخصصة لتحديد أنواع E.
faecalis E.
faccium ، و قد أظهرت نتائج ال PCR عائدية 5 عزلات لنوع E.
faecalis و عزلتين لنوعE.
faccium .
من خلال دراسة بعض الخصائص المناعية لعزلاتEnterococcous بما في ذلك البلعمة و المقاومة لمصل الدم، أظهرت النتائج إن عزلتين من E.
faecalis E كانت حساسة للبلعمة و المصل، بينما عزلة واحدة من النوع E.
faccium مقاومة لكلا الاختبارين المذكورين.
Abstract EN
Objectives : Detection of genes responsible for identification of enterococci to the species level using PCR, determination of enterococcal serum resistance and phagocytic index. Materials and methods : seven enterococcal isolates were obtained from two hundred urine samples.
After identification of enterococcal isolates by biochemical tests, all of them were subjected to PCR technique using species-specific PCR primers for E.
faecalis and E.
faecium, enterococcal serum resistance and phagocytic index were detected. Result : This study revealed that five isolates (71.43 %) reacted with ddlE.
Faecalis ; whereas the remaining (28.57 %) isolates reacted with ddlE.
faecium.
Also, revealed that phagocytic index of two E.
faecalis and one E.
faecium isolates were 27 %, 25 % and 19 %, respectively, while one E.
faecium isolate showed phagocytic index as 8 %.
Moreover, enterococcal serum resistance showed that E.
faecalis was more sensitive to the serum as compared to E.
faecium. Conclusion : Enterococcal isolates can't be easily identified to the species level by conventional or traditional methods but it can be easily identified by species-specific PCR primers ddlE.
faecalis and ddlE.
faecium.
Enterococcous faecalis tends to be more sensitive to innate immune parameters as compared to E.
faecium.
American Psychological Association (APA)
Chabuck, Zaynab A.& al-Sharrakh, Ala H.& al-Sadi, Muhammad A. K.. 2012. Identification of enterococcal species by polymerase chain reaction technique, with study of some immunological features. Medical Journal of Babylon،Vol. 9, no. 4, pp.824-832.
https://search.emarefa.net/detail/BIM-319610
Modern Language Association (MLA)
Chabuck, Zaynab A.…[et al.]. Identification of enterococcal species by polymerase chain reaction technique, with study of some immunological features. Medical Journal of Babylon Vol. 9, no. 4 (2012), pp.824-832.
https://search.emarefa.net/detail/BIM-319610
American Medical Association (AMA)
Chabuck, Zaynab A.& al-Sharrakh, Ala H.& al-Sadi, Muhammad A. K.. Identification of enterococcal species by polymerase chain reaction technique, with study of some immunological features. Medical Journal of Babylon. 2012. Vol. 9, no. 4, pp.824-832.
https://search.emarefa.net/detail/BIM-319610
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 831-832
Record ID
BIM-319610