Biological and molecular characterization of an Egyptian isolate of soybean mosaic virus in Egypt

Abstract EN

Soybean mosaic Potyviridae (SbMV) was isolated from naturally infected soybean plants cvs.

Giza 111 and Giaza 35 grown at Giza Research Station Farm, Agricultural Research Centre, Giza.

The inculated indicator plants gave severe mosaic on soybean cv.

Giza 111, mosaic and malformation on cv.

Giza 35, mild mosaic on cv.

Giza 22, yellow mild mosaic on cv.

Giza 83, vein clearing and mild mosaic on phaseolus vulgaris cvs Contender.

Chlorotic local lesion on Chenopodium quinoa.

The virus was transmitted in non-persistent manner by aphids.

Electron Microscopy showed aggregates of flexuous virus particles, degenerated mitochondria and inclusions appeared as pinwheel in cytoplasm of infected soybean leaves.

Dot blot immunoassay (DBIA) and indirect enzyme linked immunosorbent assays (ELISA) were used as serological methods for detection and confirmation of SbMV isolate in infected tissues.

A reverse transcription–polymerase chain reaction (RT-PCR) method was used to detect of local isolate of SbMV in infected plants using specific oligonucleotides primer.

A major RT-PCR fragment (~340 bp) from the coat protein gene of the SbMV genome was detected.