The KRAS strip assay for detection of KRAS mutation in Egyptian patients with colorectal cancer (CRC)‎ : a pilot study

Abstract EN

Background : Epidermal growth factor receptor (EGFR) and its downstream factors KRAS and BRAF are mutated in several types of cancer, affecting the clinical response to EGFR inhibitors.

Mutations in the EGFR kinase domain predict sensitivity to the tyrosine kinase inhibitors gefitinib and erlotinib in lung adenocarcinoma, while activating point mutations in KRASand BRAF confer resistance to the anti-EGFR monoclonal antibody cetuximab in colorectal cancer.

The development of new generation methods for systematic mutation screening of these genes will allow more appropriate therapeutic choices.

Purpose : Detection of KRAS mutation in Egyptian colorectal cancer (CRC) patients by the KRAS StripAssay.

Methods : Examination of 20 colorectal cancer (CRC) patients is done to detect KRAS mutations by KRAS StripAssay.

For the StripAssay, a mutant-enriched PCR was followed by hybridization to KRAS-specific probes bound to a nitrocellulose strip.

Results : Among 20 patients, KRASmutations were identified in 80 % of patients by theKRAS Strip-Assay.

Conclusions : Our preliminary results suggest that KRAS StripAssay is an alternative to protocols currently in use for KRAS mutation detection.