Development and validation of indirect visible spectrophotometric method for doxepin and dothiepinin pureandthe tablet dosage forms
Other Title(s)
التطوير و التحقق من صحة الطريقة الطيفية المرئية الغير مباشرة للأشكال الدوائية للمستحضرات الدوائية Dothiepinin و Doxepin
Joint Authors
Revanasiddappa, Hosakere Doddarevanna
Mallegowda, Shiramahalli Mallegowda
Source
Jordan Journal of Pharmaceutical Sciences
Issue
Vol. 6, Issue 1 (31 Jan. 2013), pp.1-8, 8 p.
Publisher
University of Jordan Deanship of Academic Research (DAR)
Publication Date
2013-01-31
Country of Publication
Jordan
No. of Pages
8
Main Subjects
Pharmacy, Health & Medical Sciences
Topics
Abstract AR
هدفت هذه الدراسة إلى تطوير طريقة طيفية و سريعة و دقيقة و حساسة و بسيطة للتقدير الكمي لـ Doxepin و Dothiepinin في كلا الشكلين الدوائيين (النقية، و الأقراص الدوائية).
تستند هذه الطريقة على أكسدة Doxepin و Dothiepinin في وسط حمضي متبوع بتفاعل مع الكريستال بنفسجي اللون الذي يظهر امتصاص الحد، و أقصاه 5900 نانو متر.
تم إتباع قانون Beer في نطاق تركيز 65-0 مل في جميع المستحضرات الدوائية، و تتراوح نسبة الشفاء ما بين 100.02-99.26% بالنسبة لـ.
Dox و 100.36-99.64% لـ .
Dot.
و كان معامل الانحدار 0.998 و 0.996 بالنسبة لـ.
Dox و Dot على التوالي.
و تم حساب حدود الكشف و المعايير الكمية وفقا لمبادئ ICH التوجيهية ثم تطبيق الطريقة المطورة بنجاح لتحديد Dox و Dot في أشكال دوائية.
Abstract EN
The aim of the present work is to develop a simple, sensitive, accurate and rapid visible spectrophotometric method for the quantitative estimation of doxepin and dothiepin in both pure and tablet dosage forms.
The developed method is based on the oxidation of DOX and DOT by Ce (IV) inan acid medium followed by reacting it with leuco crystal violetto form a violetcoloreddye which showed absorption maxima (λmax) at 590 nm.
Beer's law was obeyed in the concentration range of 0-60μg mL-1in both the drugs.
The recovery ranged from 99.26-100.02 % for DOX and from 99.64–100.36 % for DOT.
The regression coefficient was found to be 0.998 and 0.996for DOX and DOT, respectively.
The limits of detection (LOD) and quantification (LOQ) values are calculated according to ICH guidelines.
The method developed was successfully applied for the determination of DOX and DOT in dosage forms.
American Psychological Association (APA)
Revanasiddappa, Hosakere Doddarevanna& Mallegowda, Shiramahalli Mallegowda. 2013. Development and validation of indirect visible spectrophotometric method for doxepin and dothiepinin pureandthe tablet dosage forms. Jordan Journal of Pharmaceutical Sciences،Vol. 6, no. 1, pp.1-8.
https://search.emarefa.net/detail/BIM-319963
Modern Language Association (MLA)
Revanasiddappa, Hosakere Doddarevanna& Mallegowda, Shiramahalli Mallegowda. Development and validation of indirect visible spectrophotometric method for doxepin and dothiepinin pureandthe tablet dosage forms. Jordan Journal of Pharmaceutical Sciences Vol. 6, no. 1 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-319963
American Medical Association (AMA)
Revanasiddappa, Hosakere Doddarevanna& Mallegowda, Shiramahalli Mallegowda. Development and validation of indirect visible spectrophotometric method for doxepin and dothiepinin pureandthe tablet dosage forms. Jordan Journal of Pharmaceutical Sciences. 2013. Vol. 6, no. 1, pp.1-8.
https://search.emarefa.net/detail/BIM-319963
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 7
Record ID
BIM-319963
