Constructing and building-up a home made semi-automated ion chromatography

Abstract EN

Ion Chromatography (IC) is a chromatographic separation and measurement of ionic species.

This technique combines an ion – exchange chromatographic separation with simultaneous conductometric detection for the determination of anions, cations and other ionic species.

Single – column IC system equipped with a conductivity detector and with a home-made flow cell 12.5 μl sample volume was constructed and build-up from simple and inexpensive parts readily available in our analytical laborites .The approach of single-column IC instrumentation depend on the small differences in the conductivity between sample ions and the prevailing eluent .To amplify these difference , low capacity exchanger are used for the home-made separation column (3 mm ID and 300 mm length ) which permit elution with low electrolyte concentration. The home-made single column IC has been applied to separate and determination of 25 μl of injecting, anions (F- , Cl-, Br-, NO2-, NO3-, SO4-2 and PO4-2) and cations (Li+, Na+, K+, NH4+).

The accuracy of the method was examined by performing a recovery experiments using standard additions method.

The results were compared and good agreement was obtained (94.28- 100.58) %.