Spectrophotometric determination of pyridoxine hydrochloride via complexation with Fe (III)‎ in pharmaceutical and environmental wastewater samples

Abstract EN

To develop spectrophotometric method for the determination of pyridoxine hydrochloride in commercial dosage forms and industrial wastewater samples.

The method is based on the chelation of the drug with Fe III to form red colored metal chelate at room temperature which absorbs maximally at 465 nm.

Beer s law is obeyed over the concentration range of 2-28 µg / ml with molar absorptivity and Sandell s sensitivity of 0.534 x 104 l / mol.

cm and 0.035µg / cm2 respectively, relative standard deviation (RSD) is less than 2.0 (n = 10).

The method is applied successfully for determination of pyridoxine hydrochloride in some pharmaceutical formulations (tablets and injection) and industrial wastewater samples.

A statistical comparison of these results with those of official method using (t and F) values at 95 % confidence level shows good agreement and indicates no significant difference in the precision.

So that the proposed method can be used as a routine quality control for determination of pyridoxine hydrochloride in pure form, pharmaceutical formulations and industrial wastewater samples.