The antioxidant effect of different doses of chromium chloride supplementation in rats

Abstract EN

The present work was done to elucidate the possible protective and anti-oxidant effect of low and high doses of chromium chloride supplementa-tion in rats.

The experiment was carried out on 63 adult male Sprague Dawley rats weighing 150-200 g.

Rats were randomly divided into three equal groups each had 21 rats as follow ; group I (control), group II (low dose) of chromium chloride (0.8 μg / ml drinking water) and group III (high dose) of chromium chloride (4 μg / ml drinking water).

Blood samples were collected after 2, 4 and 6 weeks for assessment of erythrocytes antioxi-dants.

Glutathione (GSH) content and the activity of superoxide dismutase (SOD) and catalase (CAT) as well as glutathione S-transferase (GST) were elevated by both doses of Cr than control during the whole period of expe-riment.

Moreover, an increase in glutathione peroxidase (GSH-px) activity was observed after 4 and 6 weeks in chromium low dose (CLD)-treated group and during whole period of the experiment in chromium high dose (CHD)-treated group.

It could be concluded that, administration of chromium at high dose (CHD) reveals the potent antioxidant.