Spectrophotometric method for determination of ammonia in industrial water using ninhydrin
Other Title(s)
استخدام الطرق الطيفية لتحديد الأمونيا الموجودة في المياه الصناعية باستخدام النينهيدرين
Dissertant
Thesis advisor
Comitee Members
al-Suud, Yasin Ahmad
al-Mumani, Idris Falih
al-Digs, Yahya S. A.
University
Al albayt University
Faculty
Faculty of Sciences
Department
Department of Chemistry
University Country
Jordan
Degree
Master
Degree Date
2012
English Abstract
A simple, accurate and precise spectrophotometric method is presented for the determination of ammonia based on the formation of a deep red color product with ninhydrin in the presence of sodium hydroxide with an absorption maximum at 508 nm.
The reaction proceeds quantitatively at pH 10.0 at room temperature in 20 min.
The calibration curve is linear over the range of 2 x l04 - 1.4 x l0-3 mol \ L and is described by the regression equation A = 1069 C+ 0.109 with a regression coefficient (R2) of 0.994 (n = 7).
The calculated molar absorptivity and Sandell sensitivity values are 1.069xl03 L / mol / cm and 62.88 mol /cm2, respectively.
The limits of detection (LOD) and quantification (LOQ) are calculated to be 1.2xl0"5 and 3.6 x l0 -5 mol / L, respectively.
The intra-day accuracy expressed as relative error was less than 0.5 % with precision (RSD) ranging from 0.49 to 1.55 %.
The inter-day accuracy ranged (RSD) from 0.49 to 1.9 % with a relative error was less than 1.5 %.
The method was successfully applied for determination of the ammonia in industrial wastewater.
Accuracy was also checked by placebo blank and synthetic mixture determined besides a recovery study via standard addition procedure.
The variables affecting the reactions were carefully investigated and the conditions were optimized.
The stoichiometry of the reactions were determined molar combining ratio of 1 : 2 between ammonia and ninhydrin, and the reactions pathways were postulated.
The initial rate and fixed time methods were utilized for constructing the calibration graphs for the determination of ammonia concentration.
Main Subjects
Topics
No. of Pages
95
Table of Contents
Table of contents.
Abstract.
Chapter One : introduction.
Chapter Two : experimental.
Chapter Three : results and discussion.
Chapter Four : conclusions.
References.
American Psychological Association (APA)
al-Zbun, Khayriyah Fiddi. (2012). Spectrophotometric method for determination of ammonia in industrial water using ninhydrin. (Master's theses Theses and Dissertations Master). Al albayt University, Jordan
https://search.emarefa.net/detail/BIM-321363
Modern Language Association (MLA)
al-Zbun, Khayriyah Fiddi. Spectrophotometric method for determination of ammonia in industrial water using ninhydrin. (Master's theses Theses and Dissertations Master). Al albayt University. (2012).
https://search.emarefa.net/detail/BIM-321363
American Medical Association (AMA)
al-Zbun, Khayriyah Fiddi. (2012). Spectrophotometric method for determination of ammonia in industrial water using ninhydrin. (Master's theses Theses and Dissertations Master). Al albayt University, Jordan
https://search.emarefa.net/detail/BIM-321363
Language
English
Data Type
Arab Theses
Record ID
BIM-321363
