Effect of different doses of sodium ortho vandate supplementation on DNA and sialic acid in rats

Abstract EN

The present study was conducted to investigate the biochemical effect of low and high doses of sodium ortho- vandate supplementation on some blood and organ parameters in rats.

The experimental study was carried out on 63 adult male rats weighing 150-200 gm.

Rats were ran- domly divided into three equal groups each one comprised 21 rats and classified as follow : Group I (control group), group II (low dose of so-dium ortho-vanadate group received 0.2 mg / ml sodium chloride solution 80 mM) and group III (high dose of sodium ortho-vanadate group re-ceived 0.6 mg / ml sodium chloride solution 80 mM) via drinking water. Blood samples and tissue specimens were collected after 2, 4 and 6 weeks from the onset of sodium ortho-vanadate supplementation.

The obtained results revealed that a reduction (P < 0.05) in serum total sialic acid (TSA) concentration and the percentage of liver DNA fragmentation were observed after 4 and 6 weeks of low dose of sodium ortho-vanadate, 0.2 mg / ml (VLD) administration as compared with control.

Meanwhile a marked increase (P < 0.05) in TSA and percentage of liver, heart DNA fragmentation were recorded after administration of sodium ortho-vandate at high dose, 0.6 mg / ml (VHD) all over the period of the experi-ment.

In addition, an elevation in heart and liver DNA content and reduc-tion in the percentage of heart DNA fragmentation were observed after 6 weeks in VLD- treated group.

From the obtained results it could be con-cluded that, low dose of sodium ortho-vanadate has antioxidant and anti-apoptotic effect, while high dose of sodium ortho-vanadate has pro-oxidant and apoptotic effect.