Ciprofloxacin drug residues in table eggs

Abstract EN

The present study was conducted for analysis of ciprofloxacin in the eggs and laying hen's tissues throughout field and experimental studies and their accompanied changes in some serum biochemistry as well as some, liver and kidney functions tests.

For the field study, eggs were collected from local markets at Ismailia where it evaluated for ciprofloxacin residues.

While in the experimental study, laying hens were administered with therapeutic dose of ciprofloxacin (10 mg / kg / BW for 5 successive days) in drinking water and then its residues was investigated in both eggs (white and yolk) and the tissues (muscle, kidney and liver) using enzyme-linked immunosorbent assay (ELISA) during treatment period and for 10 days post-treatment.

The result indicated that, 20 out of 45 (44.44 %) randomly collected egg samples had ciprofloxacin residues that was higher than the levels recorded in hens treated with recommended therapeutic doses in our experimental studies.

The minimum, maximum and mean values ppb (μg / kg) for the ciprofloxacin residues were 45.6, 254.2 and 149.9 for the yolk samples and 51.2, 281.3 and 166.25 for the white samples.

Egg white had residual level more than egg yolk.

The experimental studies revealed that, The mean values of the ciprofloxacin residues during 5 days of the oral treatment were (6.88 ± 0.87), (10.20 ± 1.06), (20.72 ± 1.37), (50.25 ± 2.36), (159.15 ± 4.20) for the egg yolk and (14.96 ± 1.28), (21.81 ± 1.55), (49.72 ± 2.19), (88.46 ± 3.13), (169.58 ± 4.34) for the egg white respectively.

No ciprofloxacin residue was detected in both egg yolk and white at 7 day post-oral treatment.

The mean values of ciprofloxacin residue in in meat samples post-oral administration were (34.11 ± 2.09), (27.16 ± 1.74), (51.56 ± 1.29), (07.72 ± 0.92) ; for liver the tracing of residues extend to the eight day where the values were (527.25 ± 3.75), (501.36 ± 3.40) ),(81.14 ± 3.08), (76.66 ± 2.91), (70.25 ± 2.79), (61.21 ± 2.69), (11.45 ± 2.25) and (15.71 ± 2.37) ; and (541.21 ± 4.64), (571.15 ± 4.41) , (514.55 ± 4.07), (525.73± 3.67), (43.77± 3.22), (72.25± 2.82), (56.25 ± 2.71), (26.25 ± 2.71) in kidney tissue samples after 8 days.

No ciprofloxacin residue was detected in the meat at 5 days post-oral administration and in both liver and kidney samples at 9 days post-oral administration.

Ciprofloxacin administration caused a significant increase in plasma AST and ALT levels and non-significant decrease in total plasma protein and albumen level in treated birds 5 days post-oral administration.

It could be concluded that, the ciprofloxacin residue in the ready eggs for marketing was higher than those recorded in eggs at 2 days post oral administration for both egg yolk and white.

The therapeutic treatment resulted a ciprofloxacin residue in eggs until 6 days post-oral administration and in muscles until 4 days post-oral administration and until 8 days post-oral administration for both liver and kidney tissues, also residue was found to be higher in liver and kidney tissues than muscles until 8 days post treatment period which may pose public health risks to consumers.

Moreover, the therapeutic treatment with ciprofloxacin negatively affects the liver function.

Therefore, strict rules and regulations should be adopted for the use of ciprofloxacin in laying hens and periodical checkup of ciprofloxacin residues in table eggs and hen's tissues is advised that is to protect the consumer health.