Analysis of short tandem repeats (STRs)‎ of Y-chromosome among the Arab Iraqi population

Dissertant

al-Majali, Ruba Muhammad Isa

Thesis advisor

al-Mahasinah, Ihsan Ali

Comitee Members

al-Khawlidah, Rida Abd Allah Urayq Shibli
Irshaid, Fawzi
Mansi, Kamal M.

University

Al albayt University

Faculty

Faculty of Sciences

Department

Department of Biological Sciences

University Country

Jordan

Degree

Master

Degree Date

2011

English Abstract

The Short Tandem Repeats (STRs) of the human Y chromosome were analyzed for 17 loci in blood samples collected from 29 unrelated males from the area of Iraq (whom live in Jordan).

The allele patterns were detected using ABI310 genetic analyzer for the following 17 STR’s DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385a, DYS385b, DYS393, DYS391, DYSH4 DYS439, DYS635, DYS392, DYS437, DYS438 and DYS448.

When we compared the allelic frequencies for the population studied we found that maximum allele frequencies were 89, 6 % for DYS392, while minimum allele frequencies were 3 % for the loci : DYS : 390, 3891, 385a, 385b, 393, 439, 635, 437, 448, and H4.

The genetic homogeneity differs between the six subpopulations as follows : 52 %, 57 %, 53 %, 72 %, 54 %, 58 % for Nagaf, Baghdad, Qadesia, Basra, Babel, and Karbala ; respectively, this suggest that a high homogeneity for the whole Jra population was 54 % Gene diversity of Iraq population seemed to be high = 0,59.

This result confirms the low homogeneity of this population, this value have been took by the Allele diversity for each locus, and then the Total Gene Diversity, For each when we take each subpopulation alone, the allele diversity of Nagaf, Baghdad, Qadesia, Basra, Babel, Karbala was equal to 0.51, 0.54, 0.06, 0.79, 0.85, and 0.73 respectively, but Qadesia population recorded a lowest gene diversity (0.06).

The Minimal Haplotype composed of two Y-STRs: DYS393, DYS392 which were observed in Iraq population samples and the model Haplotype of 17-YSTRs.

The model Haplotype suggested in this study is fully harmonious with Arabian Haplotype model such as Arabian gulf countries, Saudi Arabia, Kuwait, Qatar, Oman, United Arab emirates, Oman, Bahrain, Yemen, Iraq, Syria and Lebanon with similarity ratio equal to 65 %, (Rajab, 2008) and Arab of north Africa with similarity ratio equal 71 %% (Sayyed ; 2008),this result was concluded by compare the modal Haplotype of Iraqi population extracted from this study and compare it with the modal Haplotype lines in previous studies achieved in Arab countries by other researchers.

Moreover we can compare it with Beer Alsabaa Bedouin population (Abomsimir, 2010) with similarity ratio equal to 71 % and Jordan valley (Mohamadeen, 2011) with similarity ratio equal to 53 %, And northern Jordan (Mouet, 2008) with similarity ratio equal to 53 %.

Table below represent a Comparison of Modal Haplotype of Iraq population with other populations.

In conclusion the data of allele frequency for the whole loci, were considered a primary step to establish DNA data base and a good tool to identify Iraq population.

Main Subjects

Biology

Topics

• Iraq

No. of Pages

91

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-321517