Spectrophotometric assay of metoclopramide hydrochloride in pharmaceutical preparations via arsenazo III-cerium (III)‎ reaction

Abstract EN

A simple, rapid, accurate and precise spectrophotometric method is proposed for determining metoclopramide–hydrochloride (MCPH) in pure form and in pharmaceutical preparations .The method is based on oxidation - reduction reaction between metoclopramide-hydrochloride and cerium (IV) ion, then the subsequent reaction of cerium (III) and arsenazo III reagent in acidic medium to produce a blue-violet complex which is stable, water-soluble and has a maximum absorption at 654nm with a molar absorptivity of 9.36 x 104 l.mol-1.cm-1 and Sandell's sensitivity of 0.0038 μg.cm-2.

Beer's law is obeyed in concentration range from 1-30 μg metoclopramide-hydrochloride in final volume of 25 ml, (0.04-1.2) ppm with a relative standard deviation (RSD) (±1.80 %-±3.98 %) and the limit of detection (LOD) is 0.0095 μg / ml and the limit of quantitation (LOQ) is 0.0317 μg / ml.

The method is suitable for the determination of metoclopramide -hydrochloride in the presence of other ingredients that are usually present in dosage forms and the recoveries are obtained in the range of 97.6-101.0 %.

The proposed method has been applied successfully to the determination of the intended compound in its pharmaceutical preparations (tablet and injection).