Detection of A, A-mutations of g6pd gene on molecular level in iraqi population
Other Title(s)
الكشف عن طفرات A, A-المصاحبة لأنزيم نازع هيدروجين الكلوكوز-6-فوسفات (G6PD) على المستوى الجزيئي باستعمال تقنية PCR RFLP في عينات محلية
Joint Authors
al-Jumayli, Isam Fadil A.
al-Tamimi, Rana Adil
al-Yasin, Ahmad
al-Murrani, Walid
Source
Iraqi Journal of Biotechnology
Issue
Vol. 11, Issue 2 (31 Dec. 2012), pp.357-368, 12 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2012-12-31
Country of Publication
Iraq
No. of Pages
12
Main Subjects
Topics
Abstract AR
تضمنت الدراسة استخلاص الدنا من 71 عینة مصابة سرسریا بنقص نازع هیدروجین الكلوكوز -6- فوسفات (G6PD) و 85 حالة من الأصحاء ظاهریا ثم أخضعت هذه العینات لتفاعل التضاعف التسلسلي (PCR) بعدها تم استعمال أحد الطرق المعتمدة (RFLP) و ذلك باستعمال أنزیمات قاطعة (Nla III and Fok I) للكشف عن الطفرتین قید الدراسة.
بینت نتائج الدراسة الحالیة أن نسبة الإصابة بمرض نقص نازع هیدروجین الكلوكوز -6- فوسفات في العراق هي 7.2 % و أن الطفرة نوع A قد تسببت ب 4.2 % من مجمل حالات الإصابة في حین لم تسجل أي إصابة بالطفرة من نوع A-.
Abstract EN
The study involved the extraction of deoxyribonucleic acid (DNA) from 71 samples of random G6PD patients and 85 samples apparently healthy individuals from different Iraqi populations respectively, which was then amplified by polymerase chain reaction (PCR) and later subjected to digestion by restriction enzymes (Nla III and Fok I) to create restriction fragment length polymorphism (RFLP) to enable the detection of mutation that caused G6PD deficiency namely A and A-.
The results of the current study showed that Iraqis were affected by G6PD deficiency in a percentage 7.2%and showed that the affected cases were attributed to A mutation in 4.2%while 0%was recorded for A- mutation.
American Psychological Association (APA)
al-Tamimi, Rana Adil& al-Jumayli, Isam Fadil A.& al-Yasin, Ahmad& al-Murrani, Walid. 2012. Detection of A, A-mutations of g6pd gene on molecular level in iraqi population. Iraqi Journal of Biotechnology،Vol. 11, no. 2, pp.357-368.
https://search.emarefa.net/detail/BIM-322971
Modern Language Association (MLA)
al-Tamimi, Rana Adil…[et al.]. Detection of A, A-mutations of g6pd gene on molecular level in iraqi population. Iraqi Journal of Biotechnology Vol. 11, no. 2 (2012), pp.357-368.
https://search.emarefa.net/detail/BIM-322971
American Medical Association (AMA)
al-Tamimi, Rana Adil& al-Jumayli, Isam Fadil A.& al-Yasin, Ahmad& al-Murrani, Walid. Detection of A, A-mutations of g6pd gene on molecular level in iraqi population. Iraqi Journal of Biotechnology. 2012. Vol. 11, no. 2, pp.357-368.
https://search.emarefa.net/detail/BIM-322971
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 267-268
Record ID
BIM-322971