The clinical and histopathological effect of chitosan applied locally on the healing of experimentally induced teat fistula in goats

Abstract EN

The clinical and histopathological the effect of chitosan applied locally on the healing of experimentally induced teat fistula in goats.

Sixteen local breed goats have been used, aged range between (1.2-2) years with body weight (30 + -5) kg.

Animals were randomly allotted into two equal groups.

An artificial teat fistula of one cm.

length was done for all animals under the effect of local analgesia and deep sedation.

The first group, fistula was treated with chitosan 0.5gm and then closed by suture ; while the second group (control one), fistula was closed routinely "via suture without any addition.

After daily clinical follow-up, mild local reaction include local redness and swelling of the operative site and systemic mild hyperthermia, decrease ruminal contraction, increase respiratory rate.

Histopathological examinations were performed on all animals.

Eight animals used for each group : (2 for each period).

Sections were stained with hematoxylin–eosin stain.

Results of Histopathological examination in treated group reflected the presence of large numbers of neutrophils In the first three days postoperatively, granulation tissue were seen in the sixty days which converted to cellular connective tissue in the ninth day with congested blood vessels.

In addition there are lymphocytic aggregations, arrangement of collagen fibers and formation of new epidermis during the 12th.

Days.

In conclusion we can say that chitosan play an important role in enhancing repair of teat fistula.