Effect of P. aeruginosa metabolic products on some hematological, biochemical and histological parameters of male rats

Abstract EN

The present study was designed to prove the toxic effects of Effect of P.

aeruginosa metabolic products on some hematological, biochemical and histological on male rats Forty mature male rats, their age average was about (3-3.5) months and their weight average was about (200-225) gm, were randomly divided into 2 equal groups: treatment group (T) were drenched orally by four concentration of P.

aeruginosa metabolic products (5,50,500,5000) μl/kg body weight, five animals for each concentration and control treated orally by normal saline in the same concentrations above, also five animals each.

The animal treated with one single dose of the previously describe concentrations then left for 15 days.

Animals of different groups were sacrificed under light anesthesia one day after the end of treatment and blood samples were taken to determine some physiological, biochemical parameters, also the liver and kidneys samples.

The results revealed many changes due to treatment fluctuated according to the type of studied parameter, as follows: significance increase of WBCs count, serum ALT activity, serum AST activity, serum sugar, while hemoglobin (Hb) and serum triglyceride (TG) concentration shows significance decrease.

Also the microscopic examination of the organs shows many histopathological changes due to the treatment such as hemorrhage, extension of central vein, disarrangement of sinusoid, degeneration and hepatocytes necrosis in the liver, while kidneys sections shows epithelial infiltration, simple glomerulus's extension and slight tubular degeneration .

The findings of this study showed that liver function and liver structure severely effected by P.

aeruginosa metabolic products as well as other parameters due to the treatment.