A new method for DNA isolation from bone marrow and sperms of white mice

Abstract EN

Background : A quick and easy method was developed for extraction of DNA of eukaryotes from different samples, which are bone marrow and sperms in white mice Mus musculus strain (Balb / c). Patients and Methods : this method using high salt buffer, Ethylene diemine tetracetec acid (EDTA), Trypsine, Sodium Dodecyl Sulfate (SDS), and urea without using Proteinase-K digestion or ultracentrifugation. Results : This method was successful in extracting DNA from different samples in eukaryotic and this DNA is suitable for Hind III digestion. Conclusion : Without further clean-up, the extracted DNA can be used for restriction endonuclease digestion or for numerous applications.