A new method for DNA isolation from bone marrow and sperms of white mice
Author
Source
Journal of the Faculty of Medicine Baghdad
Issue
Vol. 51, Issue 2 (31 Dec. 2009), pp.220-222, 3 p.
Publisher
University of Baghdad Faculty of Medicine
Publication Date
2009-12-31
Country of Publication
Iraq
No. of Pages
3
Main Subjects
Topics
Abstract EN
Background : A quick and easy method was developed for extraction of DNA of eukaryotes from different samples, which are bone marrow and sperms in white mice Mus musculus strain (Balb / c). Patients and Methods : this method using high salt buffer, Ethylene diemine tetracetec acid (EDTA), Trypsine, Sodium Dodecyl Sulfate(SDS), and urea without using Proteinase-K digestion or ultracentrifugation. Results : This method was successful in extracting DNA from different samples in eukaryotic and this DNA is suitable for Hind III digestion. Conclusion : Without further clean-up, the extracted DNA can be used for restriction endonuclease digestion or for numerous applications.
American Psychological Association (APA)
Ahmad, Shayma J.. 2009. A new method for DNA isolation from bone marrow and sperms of white mice. Journal of the Faculty of Medicine Baghdad،Vol. 51, no. 2, pp.220-222.
https://search.emarefa.net/detail/BIM-326831
Modern Language Association (MLA)
Ahmad, Shayma J.. A new method for DNA isolation from bone marrow and sperms of white mice. Journal of the Faculty of Medicine Baghdad Vol. 51, no. 2 (2009), pp.220-222.
https://search.emarefa.net/detail/BIM-326831
American Medical Association (AMA)
Ahmad, Shayma J.. A new method for DNA isolation from bone marrow and sperms of white mice. Journal of the Faculty of Medicine Baghdad. 2009. Vol. 51, no. 2, pp.220-222.
https://search.emarefa.net/detail/BIM-326831
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 222
Record ID
BIM-326831