Dietary administration of undennatured whey protein decrased the histopathological changes induced by ciprofloxacin in hepatic, renal and ilael tissues of mice

Abstract EN

Whey protein contains all amino acids that are necessary for normal cell growth.

This work aimed to study whether whey protein can decrease the effects induced by Ciprofloxacin.

Histological, histochemical and immunological methods were used.

Mice were classified into a control (group I) and three treated groups.

Group II was daily administrated Ciprofloxacin for 7 days.

Group III was administrated Ciprofloxacin and synergistically mice administrated whey, protein for the 7 days.

Mice of group IV were treated in the same way like as those of group III with the whey protein continue for 8 days more (whey protein was administrated 15 days in group IV instead-of 7 days in group 111).

Liver sections of group II revealed that cell boundaries of hepatocytes became unclear, blood sinusoids became narrow and Kupffer cells increased in both size and number.

PAS stain showed a reduced carbohydrate content in group II, while it showed a little improved in group TV.

A moderate improvement in the protein content was observed in this group.

Kidney sections of group II appeared with some cellular inflammations, edematous renal cells and glomeruli were shrunken.

Also a decrease in carbohydrate and protein contents was observed.

An obvious effect of the whey protein had obtained of the renal tissues in group IV, since glumeruli, renal tubules and the general histological architecture became similar to the normal.

Ileum sections revealed a thickness in the muscular is and increasing in crypts of Lieberkuhn and in fibers.

An improvement was noted in group IE and increased as the time of whey protein prolonged in group IV.

There was an observed decrease of the carbohydrate contents in both group n and group III.

This carbohydrate contents intensity was obviously increased in goblet seals and brush border of group IV.

The intensity- of proteins in the tissues of treated s was nearly as that in control.

The physiological and immunological indices reflected the same severe effect of the antibiotic.

An observed improvement was achieved in the phagocytic activity and ' the chemo kinetic index after administration of the indentured whey Protein.

Conclusion, these findings showed severe effects of the Ciprofloxacin on the histological architecture of studied organs.

After 15 days (group TV) an improvement was observed in both renal and ileal tissues but could not completely restore their normal histological architecture.

This protein obviously affected the physiological and the immunological responses, and this might be the potential factor in initiating the normalizing processes of the histological architecture structures.